Literature DB >> 2541020

Phosphoinositide hydrolysis in permeabilized SH-SY5Y human neuroblastoma cells is inhibited by mastoparan.

R J Wojcikiewicz1, S R Nahorski.   

Abstract

The effects of mastoparan on phospholipase C-catalysed phosphoinositide hydrolysis were examined in [3H]inositol-labelled human neuroblastoma SH-SY5Y cells. [3H]Inositol phosphate formation in intact cells was not altered by 20 microM mastoparan. In contrast, [3H]inositol phosphate formation in electrically permeabilized cells stimulated with guanosine 5'-[gamma-thio]triphosphate and/or carbachol was inhibited by mastoparan with half-maximal effects at approx. 3 microM. The peptide was much less effective in inhibiting stimulatory effects of Ca2+. Similar but less potent inhibitory effects were observed with the cations, neomycin and spermine, indicating that direct interaction of mastoparan with polyphosphoinositides might account for its inhibitory effects on inositol phosphate formation.

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Year:  1989        PMID： 2541020     DOI： 10.1016/0014-5793(89)81366-3

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  9 in total

1.  Characterization of a mastoparan-stimulated nucleotidase from bovine brain.

Authors:  B M Denker; P Tempst; E J Neer
Journal:  Biochem J       Date:  1991-09-01       Impact factor: 3.857

2.  Micromolar free calcium exposes ouabain-binding sites in digitonin-permeabilized Xenopus laevis oocytes.

Authors:  G Schmalzing; S Kröner
Journal:  Biochem J       Date:  1990-08-01       Impact factor: 3.857

3.  Activation of superoxide formation and lysozyme release in human neutrophils by the synthetic lipopeptide Pam3Cys-Ser-(Lys)4. Involvement of guanine-nucleotide-binding proteins and synergism with chemotactic peptides.

Authors:  R Seifert; G Schultz; M Richter-Freund; J Metzger; K H Wiesmüller; G Jung; W G Bessler; S Hauschildt
Journal:  Biochem J       Date:  1990-05-01       Impact factor: 3.857

4.  Dual effects of mastoparan on intracellular free Ca2+ concentrations in human astrocytoma cells.

Authors:  N Nakahata; H Ishimoto; K Mizuno; Y Ohizumi; H Nakanishi
Journal:  Br J Pharmacol       Date:  1994-05       Impact factor: 8.739

5.  The effects of mastoparan on the carrot cell plasma membrane polyphosphoinositide phospholipase C.

Authors:  M H Cho; Z Tan; C Erneux; S B Shears; W F Boss
Journal:  Plant Physiol       Date:  1995-03       Impact factor: 8.340

6.  Effects of the amphiphilic peptides mastoparan and adenoregulin on receptor binding, G proteins, phosphoinositide breakdown, cyclic AMP generation, and calcium influx.

Authors:  Y Shin; R W Moni; J E Lueders; J W Daly
Journal:  Cell Mol Neurobiol       Date:  1994-04       Impact factor: 5.046

7.  Mastoparan promotes exocytosis and increases intracellular cyclic AMP in human platelets. Evidence for the existence of a Ge-like mechanism of secretion.

Authors:  C P Wheeler-Jones; T Saermark; V V Kakkar; K S Authi
Journal:  Biochem J       Date:  1992-01-15       Impact factor: 3.857

8.  Neomycin is a potent secretagogue of mast cells that directly activates a GTP-binding protein involved in exocytosis.

Authors:  M Aridor; R Sagi-Eisenberg
Journal:  J Cell Biol       Date:  1990-12       Impact factor: 10.539

Review 9.  Mastoparans: A Group of Multifunctional α-Helical Peptides With Promising Therapeutic Properties.

Authors:  Carlos José Correia de Santana; Osmindo Rodrigues Pires Júnior; Wagner Fontes; Mário Sérgio Palma; Mariana S Castro
Journal:  Front Mol Biosci       Date:  2022-06-24
  9 in total

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