AIMS: To evaluate the diversity of endophytic fungi from the leaves of the common bean and the genetic diversity of endophytic fungi from the genus Colletotrichum using IRAP (inter-retrotransposon amplified polymorphism) and REMAP (retrotransposon-microsatellite amplified polymorphism) analyses. METHODS AND RESULTS: The fungi were isolated by tissue fragmentation and identified by analysing the morphological features and sequencing the internal transcribed spacer (ITS) regions and the rDNA large subunit (LSU). Twenty-seven different taxa were identified. Colletotrichum was the most commonly isolated genera from the common bean (32.69% and 24.29% of the total isolates from the Ouro Negro and Talismã varieties, respectively). The IRAP and REMAP analyses revealed a high genetic diversity in the Colletotrichum endophytic isolates and were able to discriminate these isolates from the phytopathogen Colletotrichum lindemuthianum. CONCLUSIONS: Fungi from the genus Colletotrichum are abundant in the Phaseolus vulgaris endophytic community, and the IRAP and REMAP markers can be used to rapidly distinguish between C. lindemuthianum and other Colletotrichum members that are frequently found as endophytes. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first report of the diversity of endophytic fungi present in the common bean and the use of IRAP and REMAP markers to assess the genetic diversity of endophytic fungi from the genus Colletotrichum.
AIMS: To evaluate the diversity of endophytic fungi from the leaves of the common bean and the genetic diversity of endophytic fungi from the genus Colletotrichum using IRAP (inter-retrotransposon amplified polymorphism) and REMAP (retrotransposon-microsatellite amplified polymorphism) analyses. METHODS AND RESULTS: The fungi were isolated by tissue fragmentation and identified by analysing the morphological features and sequencing the internal transcribed spacer (ITS) regions and the rDNA large subunit (LSU). Twenty-seven different taxa were identified. Colletotrichum was the most commonly isolated genera from the common bean (32.69% and 24.29% of the total isolates from the Ouro Negro and Talismã varieties, respectively). The IRAP and REMAP analyses revealed a high genetic diversity in the Colletotrichum endophytic isolates and were able to discriminate these isolates from the phytopathogen Colletotrichum lindemuthianum. CONCLUSIONS: Fungi from the genus Colletotrichum are abundant in the Phaseolus vulgaris endophytic community, and the IRAP and REMAP markers can be used to rapidly distinguish between C. lindemuthianum and other Colletotrichum members that are frequently found as endophytes. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first report of the diversity of endophytic fungi present in the common bean and the use of IRAP and REMAP markers to assess the genetic diversity of endophytic fungi from the genus Colletotrichum.
Authors: Amanda da Silva Ribeiro; Julio Cesar Polonio; Alessandra Tenório Costa; Caroline Menicoze Dos Santos; Sandro Augusto Rhoden; João Lúcio Azevedo; João Alencar Pamphile Journal: Curr Microbiol Date: 2018-01-03 Impact factor: 2.188
Authors: Amanda da Silva Ribeiro; Julio Cesar Polonio; João Arthur Dos Santos Oliveira; Ana Paula Ferreira; Leonardo Hamamura Alves; Natieli Jenifer Mateus; Claudete Aparecida Mangolin; João Lúcio de Azevedo; João Alencar Pamphile Journal: Braz J Microbiol Date: 2021-09-07 Impact factor: 2.214
Authors: Anna Maria Vettraino; Hong-Mei Li; Rene Eschen; Carmen Morales-Rodriguez; Andrea Vannini Journal: PLoS One Date: 2017-11-29 Impact factor: 3.240
Authors: Fábio de Azevedo Silva; Rhavena Graziela Liotti; Ana Paula de Araújo Boleti; Érica de Melo Reis; Marilene Borges Silva Passos; Edson Lucas Dos Santos; Olivia Moreira Sampaio; Ana Helena Januário; Carmen Lucia Bassi Branco; Gilvan Ferreira da Silva; Elisabeth Aparecida Furtado de Mendonça; Marcos Antônio Soares Journal: PLoS One Date: 2018-04-12 Impact factor: 3.240