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Literature DB >> 25408838

Aptamer-facilitated cryoprotection of viruses.

Shahrokh M Ghobadloo¹, Ana Gargaun¹, Rebecca Casselman¹, Darija Muharemagic¹, Maxim V Berezovski¹.

Abstract

Global vaccination and gene therapy programs have an urgent demand for stabilization of viral vectors at low temperature. We used a quadramer, a bridge-connected DNA tetra-aptamer to antivesicular stomatitis virus (VSV), as a viral cryoprotectant. Results showed that the tetravalent antivirus DNA aptamers protect viral activity during multiple freeze-thaw cycles, shield from neutralizing antibodies, and decrease aggregation of viral particles.

Entities: Disease Species

Keywords: Aptamer; cryoprotection; quadramer; vesicular stomatitis virus; viral stabilizers

Year: 2014 PMID： 25408838 PMCID： PMC4233356 DOI： 10.1021/ml500322h

Source DB: PubMed Journal: ACS Med Chem Lett ISSN： 1948-5875 Impact factor: 4.345

17 in total

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Authors: R Gibson; S Schlesinger; S Kornfeld
Journal: J Biol Chem Date: 1979-05-10 Impact factor: 5.157

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Authors: Pam Das
Journal: Lancet Infect Dis Date: 2004-12 Impact factor: 25.071

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Authors: Marilyn Fernandez; Mercedes Porosnicu; Dubravka Markovic; Glen N Barber
Journal: J Virol Date: 2002-01 Impact factor: 5.103

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Authors: Jean-Simon Diallo; Markus Vähä-Koskela; Fabrice Le Boeuf; John Bell
Journal: Methods Mol Biol Date: 2012

7. Vesicular stomatitis virus oncolysis is potentiated by impairing mTORC1-dependent type I IFN production.

Authors: Tommy Alain; XueQing Lun; Yvan Martineau; Polen Sean; Bali Pulendran; Emmanuel Petroulakis; Franz J Zemp; Chantal G Lemay; Dominic Roy; John C Bell; George Thomas; Sara C Kozma; Peter A Forsyth; Mauro Costa-Mattioli; Nahum Sonenberg
Journal: Proc Natl Acad Sci U S A Date: 2010-01-04 Impact factor: 11.205

8. Viral quantitative capillary electrophoresis for counting and quality control of RNA viruses.

Authors: Afnan Azizi; Gleb G Mironov; Darija Muharemagic; Mohamed Wehbe; John C Bell; Maxim V Berezovski
Journal: Anal Chem Date: 2012-10-19 Impact factor: 6.986

9. Sorting of the respiratory syncytial virus matrix protein into detergent-resistant structures is dependent on cell-surface expression of the glycoproteins.

Authors: Gary Henderson; Jillian Murray; Robert P Yeo
Journal: Virology Date: 2002-09-01 Impact factor: 3.616