| Literature DB >> 2540753 |
Abstract
When monkey COS7 cells are transfected with plasmids pSVod or pSV2-neo, a DNA structure can be detected consisting of two circular forms linked by a duplex bridge. Generation of this structure is enhanced by camptothecin, an inhibitor of DNA topoisomerase I. Generation of dual-circles in vitro, using a DNA replication system with added T-antigen, requires template DNA with an SV40 origin. Heterogeneous dual-circles can be visualized involving two initially independent molecules of different size. Implications for in vitro studies of certain types of recombination are discussed.Entities:
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Year: 1989 PMID： 2540753 DOI： 10.1016/0006-291x(89)91619-7
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575