Literature DB >> 25404304

NanoFlares for the detection, isolation, and culture of live tumor cells from human blood.

Tiffany L Halo1, Kaylin M McMahon2, Nicholas L Angeloni3, Yilin Xu4, Wei Wang4, Alyssa B Chinen1, Dmitry Malin5, Elena Strekalova5, Vincent L Cryns5, Chonghui Cheng6, Chad A Mirkin7, C Shad Thaxton8.   

Abstract

Metastasis portends a poor prognosis for cancer patients. Primary tumor cells disseminate through the bloodstream before the appearance of detectable metastatic lesions. The analysis of cancer cells in blood—so-called circulating tumor cells (CTCs)—may provide unprecedented opportunities for metastatic risk assessment and investigation. NanoFlares are nanoconstructs that enable live-cell detection of intracellular mRNA. NanoFlares, when coupled with flow cytometry, can be used to fluorescently detect genetic markers of CTCs in the context of whole blood. They allow one to detect as few as 100 live cancer cells per mL of blood and subsequently culture those cells. This technique can also be used to detect CTCs in a murine model of metastatic breast cancer. As such, NanoFlares provide, to our knowledge, the first genetic-based approach for detecting, isolating, and characterizing live cancer cells from blood and may provide new opportunities for cancer diagnosis, prognosis, and personalized therapy.

Entities:  

Keywords:  NanoFlares; cancer metastasis; diagnostic; mRNA; nanotechnology

Mesh：

Substances：

Year:  2014        PMID： 25404304      PMCID： PMC4260589          DOI： 10.1073/pnas.1418637111

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  28 in total

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  53 in total

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