Three novel single nucleotide polymorphisms of the 3-hydroxy-3-methylglutaryl coenzyme A reductase gene associated with egg-production in chicken.

Different single-strand conformation polymorphism (SSCP) patterns of the 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) gene amplified by six pairs of primers were sequenced. Comparisons among the six nucleotide sequences of three genotypes revealed three base substitutions (G-789-A, C-937-G, and A-2316-C) in the HMGCR gene. The genotypes of single nucleotide polymorphism 1 (SNP1) and SNP3 were significantly associated with the number of eggs (P < 0.01), and the different genotypes of SNP2 did not significantly influence the number of eggs. The genotypes of all three SNPs were not related to the hatching rate of fertilized eggs (P > 0.05). Of the three SNPs of the HMGCR gene, those genotypes with lower plasma very low-density lipoprotein (VLDL) concentrations had higher egg production. The 300-day egg production of the six studied haplotypes showed three levels (P < 0.01). The ADE and ACE haplotypes showed lower egg production than the other haplotypes. The ADE haplotype had the highest plasma VLDL concentration, and the ACE haplotype had the lowest plasma VLDL concentration. We concluded that specific variants of the HMGCR gene are associated with chicken egg production, and haplotypes with higher and lower plasma VLDL concentrations showed lower egg production than other haplotypes.