Insulin and insulin-like growth factor 1 stimulate proliferation of metastatic variants of colon carcinoma 26.

The proliferation rate of malignant cells in vivo is one of the important factors which affect the formation of tumor metastasis. A highly metastatic variant of mouse colon adenocarcinoma 26 (NL-17) grew more rapidly than a low-metastatic variant (NL-44) both in vitro and in vivo. The effect of growth factors on the proliferation of NL-17 and NL-44 cells was examined in serum-free medium. Among growth factors examined, human insulin and insulin-like growth factor 1 (IGF-1), which were produced by gene engineering techniques, stimulated the growth of metastatic NL-17 and NL-44 cells as determined by thymidine incorporation and cell counts. DNA synthesis and cell proliferation of the high-metastatic NL-17 was stimulated to a greater extent by insulin and IGF-1 than those of the low-metastatic NL-44. These findings suggest that circulating growth factors could enhance the formation of tumor metastasis. Scatchard analysis of [125I]IGF-1 binding to NL-17 and NL-44 showed that each cell line had an almost equal number of IGF-1 receptors (1.37 x 10(5)/cell and 1.26 x 10(5)/cell, respectively), which had similar dissociation constants (8.94 x 10(-10) M and 9.54 x 10(-10) M, respectively). Since the number and affinity of IGF-1 receptors are equivalent between low- and high-metastatic cells, the intracellular events which result in the cell growth after binding of IGF-1 may differ between NL-17 and NL-44 cells.

insulin‐like growth factor 1

multiplication stimulating activity

epidermal growth factor

fibroblast growth factor

bovine serum albumin

[125I]‐(A14)‐monoiodinated insulin

(3‐[125I]iodotyrosyl)insulin‐like growth factor‐1 [3H]thymidine, [methyl‐3H]thymidine

transforming growth factor β

Krebs‐Ringer phosphate buffer

minimum essential medium

effective concentration of non‐labeled growth factor which inhibits the specific binding of labeled growth factor by 50% of the maximum