Dilara Inan1, Murat Sayan2. 1. Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Akdeniz University Hospital, Akdeniz University, Antalya, Turkey. 2. Clinical Laboratory, PCR Unit, Kocaeli University Hospital, Kocaeli University, Izmit-Kocaeli, Turkey.
Abstract
INTRODUCTION: The aim of this study was to determine the subtype distribution of HIV-1 strains isolated from antiretroviral therapy (ART)-naive and on treatment patients in Antalya, the city of southern Turkey. MATERIALS AND METHODS: The study included 77 of 92 newly diagnosed HIV-1 positive patients of last two years (between February 2012 and June 2014). HIV-1 subtypes and circulating recombinant forms (CRFs) were identified by phylogenetic analysis of reverse-transcriptase (codon 41-238) and protease (codon 1-99) domains (~667 bp) of pol gene region in HIV-1 strains. RESULTS: Subtype B (48%, 37/77) was identified as the most common HIV-1 subtype in Antalya that similar the other Turkish patients. Non-B subtypes were followed as CRFs (39%, 30/77). Interestingly, CRF14_BG (12.9%, 10/77) was found for the first time in Antalya in contrast to previous observations in the other reports in Turkey. Also, subtype G (6.5%, 5/77) was detected more often than HIV-1 subtypes in circulation of Turkey. CONCLUSIONS: These findings may be associated with specific geographic localization of Antalya that touristic movements of city. Recognized HIV-1 subtype diversity is major challenges in the development of a globally effective HIV vaccine.
INTRODUCTION: The aim of this study was to determine the subtype distribution of HIV-1 strains isolated from antiretroviral therapy (ART)-naive and on treatment patients in Antalya, the city of southern Turkey. MATERIALS AND METHODS: The study included 77 of 92 newly diagnosed HIV-1 positive patients of last two years (between February 2012 and June 2014). HIV-1 subtypes and circulating recombinant forms (CRFs) were identified by phylogenetic analysis of reverse-transcriptase (codon 41-238) and protease (codon 1-99) domains (~667 bp) of pol gene region in HIV-1 strains. RESULTS: Subtype B (48%, 37/77) was identified as the most common HIV-1 subtype in Antalya that similar the other Turkish patients. Non-B subtypes were followed as CRFs (39%, 30/77). Interestingly, CRF14_BG (12.9%, 10/77) was found for the first time in Antalya in contrast to previous observations in the other reports in Turkey. Also, subtype G (6.5%, 5/77) was detected more often than HIV-1 subtypes in circulation of Turkey. CONCLUSIONS: These findings may be associated with specific geographic localization of Antalya that touristic movements of city. Recognized HIV-1 subtype diversity is major challenges in the development of a globally effective HIV vaccine.
Authors: Erik Billings; Richard A Heipertz; Tonka Varleva; Eric Sanders-Buell; Anne Marie O'Sullivan; Meera Bose; Shana Howell; Gustavo H Kijak; Hristo Taskov; Ivailo Elenkov; Marina Nenova; Nedialka Popivanova; Aimee Bolen Valenzuela; Otha Myles; Christian T Bautista; Merlin L Robb; Nelson L Michael; Jerome H Kim; Paul T Scott; Sodsai Tovanabutra; Julie A Ake Journal: PLoS One Date: 2019-05-28 Impact factor: 3.240