| Literature DB >> 25391804 |
Ulrike Endesfelder1, Mike Heilemann.
Abstract
Single-molecule localization-based super-resolution microscopy can be performed with regular, bright, and photostable organic fluorophores. We review a concept termed direct stochastic optical reconstruction microscopy (dSTORM), which operates conventional fluorophores as photoswitches and provides an optical resolution of ~20 nm. We introduce the principle of dSTORM, illustrate experimental schemes, and discuss approaches for data analysis.Mesh:
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Year: 2015 PMID: 25391804 DOI: 10.1007/978-1-4939-2080-8_14
Source DB: PubMed Journal: Methods Mol Biol ISSN: 1064-3745