| Literature DB >> 25391793 |
Francesco Pampaloni1, Roli Richa, Nariman Ansari, Ernst H K Stelzer.
Abstract
We provide a detailed protocol for a three-dimensional long-term live imaging of cellular spheroids with light sheet-based fluorescence microscopy. The protocol allows the recording of all phases of spheroid formation in three dimensions, including cell proliferation, aggregation, and compaction. We employ the human hepatic cell line HepaRG transfected with the fusion protein H2B-GFP, i.e., a fluorescing histone. The protocol allows monitoring the effect of drugs or toxicants.Entities:
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Year: 2015 PMID： 25391793 DOI： 10.1007/978-1-4939-2080-8_3
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745