Parathyroid hormone stimulates prostanoid formation in mouse calvarial bones.

Bovine parathyroid hormone (bPTH 1-34) caused a time- and dose-dependent enhanced formation of the two prostanoids PGE2 and 6-keto-PGF1 alpha in cultured neonatal mouse calvarial bones, with threshold for action at 0.1 nmol/l. The PGE2 response to PTH was completely blocked by indomethacin, but insensitive to calcitonin. By contrast, indomethacin was without effect on 45Ca release induced by PTH. The PTH analogues (Nle 8, 18, Tyr 34)-bPTH (3-34) amide and (Tyr 34)-bPTH (7-34) amide, which are putative PTH antagonists, did not affect basal production of PGE2, nor did the analogues affect bPTH 1-34 induced PGE2 formation. The data show that PTH stimulates prostanoid formation in mouse bone cells and that this response is not directly linked to PTH-induced bone resorption.