| Literature DB >> 25388116 |
Andrew Haydock1, Monica Terrao, Aarthi Sekar, Gowthaman Ramasamy, Loren Baugh, Peter J Myler.
Abstract
High-throughput sequencing of cDNA copies of mRNA (RNA-seq) provides a digital read-out of mRNA levels over several orders of magnitude, as well as mapping the transcripts to the nucleotide level. Here we describe an RNA-seq approach that exploits the 39-nucleotide mini-exon or spliced leader (SL) sequence found at the 5' end of all Leishmania (and other trypanosomatid) mRNAs.Entities:
Mesh：
Substances：
Year: 2015 PMID： 25388116 DOI： 10.1007/978-1-4939-1438-8_12
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745