Studies on the production of Digitalis cardenolides by plant tissue culture I. Determination of digitoxin and digoxin contents in first and second passage calli and organ redifferentiating calli of several Digitalis species by radioimmunoassay.

With first and second passage calli induced from seedlings of Digitalis purpurea, D. lanata, D. lutea, D. mertonensis, D. ambigua and D. ferruginea Gigantea and those induced from leaf discs of D. purpurea and D. lanata, the contents of digitoxin and digoxin equivalents were assayed and compared with the contents involved in the inocula. Although the total contents of digitoxin and digoxin equivalents in the first passage calli induced from seedlings varied between zero and nine times as high as in the original seedlings, those in the second passage calli were almost undetectable. The total contents of digitoxin equivalents in the first passage calli induced from leaf discs of D. purpurea were approximately equal to those in the original leaf discs, but those in the second passage calli were less than those in the inocula. In the first passage calli induced from leaf discs of D. lanata, the total contents of digitoxin equivalents decreased but those of digoxin equivalents slightly increased. However, in the second passage calli, the amounts of both cardenolides decreased. Root-forming calli accumulated no more digitoxin nor digoxin equivalents than completely dedifferentiated calli. However, shoot-forming calli accumulated considerable amounts of cardenolides, which were assayed as digitoxin and digoxin equivalents by radioimmunoassay.