Literature DB >> 2538512

Monocyte-lymphocyte discrimination in a new microtitre-based adhesion assay.

P M Bath1, R F Booth, D G Hassall.   

Abstract

A new, rapid and reproducible method is described for the quantification of in vitro monocyte adherence in microtitre 96-well ELISA plates. The assay is based on the measurement of the myeloperoxidase activity in monocytes that have adhered to either plastic or bovine/porcine aortic endothelial cells. The assay shows a linear response to monocyte concentrations over the range 5 x 10(4)-5 x 10(5) cells/well. The method provides good discrimination between monocyte and and lymphocytes and thus allows a study of mononuclear cell populations to be made. The assay works equally well with polymorphonuclear cells. The coefficients of variation are: intra-assay, 7%; interassay, 13%.

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Year:  1989        PMID： 2538512     DOI： 10.1016/0022-1759(89)90053-7

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  10 in total

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4.  Sulphatides trigger polymorphonuclear granulocyte spreading on collagen-coated surfaces and inhibit subsequent activation of 5-lipoxygenase.

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5.  Increased adhesion of human monocytes to IL-4-stimulated human venous endothelial cells via CD11/CD18, and very late antigen-4 (VLA-4)/vascular cell adhesion molecule-1 (VCAM-1)-dependent mechanisms.

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6.  Induction of adherence and degranulation of polymorphonuclear leukocytes: a new expression of the invasive phenotype of Shigella flexneri.

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Journal:  Infect Immun       Date:  1996-03       Impact factor: 3.441

7.  The effect of nitric oxide-donating vasodilators on monocyte chemotaxis and intracellular cGMP concentrations in vitro.

Authors:  P M Bath
Journal:  Eur J Clin Pharmacol       Date:  1993       Impact factor: 2.953

8.  Surface molecules involved in the adherence of recombinant interferon-gamma (rIFN-gamma)-stimulated human monocytes to vascular endothelial cells.

Authors:  J Wang; H Beekhuizen; R van Furth
Journal:  Clin Exp Immunol       Date:  1994-02       Impact factor: 4.330

9.  Studies on the interaction of leucocytes and the myocardial vasculature. I. Effect of hypoxia on the adherence of blood granulocytes.

Authors:  A Pietersma; N de Jong; W Sluiter; J F Koster
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10.  Photodynamic treatment of human endothelial cells promotes the adherence of neutrophils in vitro.

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  10 in total

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