Application of Pseudomonas flava WD-3 for sewage treatment in constructed wetland in winter.

Recently, constructed wetland was applied for sewage treatment globally due to its high efficiency and relatively low investment. However, operation of many constructed wetlands in cold winter is quite difficult due to the inhibition effect of low temperature. The objective of this experiment is to study the sewage treatment efficiency of Pseudomonas flava WD-3 in the integrated vertical-flow constructed wetland (IVCW) during winter with different dosages (bacterial suspension concentration: 4.575×10(8 )mL(-1)). Two treatments were designed, inoculation of P. flava WD-3 with different dosages and the control without bacterium incubation. A simplified Monod model was applied to simulate and evaluate the pollutant removal efficiency of this bacterial strain with respect to its dosages. Results indicated that P. flava WD-3 could degrade organic pollutants, nitrogen, and phosphorus nutrients from wastewater effectively. The optimal dosage of this strain was 6.0%, and the removal rates of chemical oxygen demand (COD), ammonium nitrogen (NH4+-N), and total phosphorous (TP) were 85.82-87.00%, 73.91-84.18%, and 82.04-87.00%, respectively. Furthermore, the average removal efficiencies of COD, NH4+-N, and TP were 1.46, 1.49, and 1.76 times, respectively, than the control. The simplified Monod model accurately predicted the pollutant removal efficiency of P. flava WD-3 in the IVCW system in winter.