T Siegemund1, U Scholz, R Schobess, A Siegemund. 1. T. Siegemund, MVZ Lab Dr. Reising-Ackermann and Colleagues, Center of Coagulation Disorders, Strümpellstr. 40, 04289 Leipzig, Germany.
Abstract
UNLABELLED: Clot waveform analysis extends the interpretation of aPTT measurement curves. The curve is mathematically processed to obtain information about fibrin formation kinetics including semiquantitative determination of thrombin, prothrombinase and tenase activity. PATIENTS, METHOD: In this study the feasibility of clot waveform analysis for monitoring of haemophilia A was investigated using blood samples from healthy controls as well as haemophilia A patients under various clinical conditions. RESULTS: Thrombin, prothrombinase and tenase activity show a high correlation to factor VIII levels. Tenase activity was found to exhibit a linear relationship to factor VIII levels over a very large concentration range and was able to discriminate patients with severe, moderate and mild haemophilia. CONCLUSION: Clot waveform analysis is an easy, fast and cheap method to access disturbances in clot formation and can be done without any additional measurements beside an aPTT.
UNLABELLED: Clot waveform analysis extends the interpretation of aPTT measurement curves. The curve is mathematically processed to obtain information about fibrin formation kinetics including semiquantitative determination of thrombin, prothrombinase and tenase activity. PATIENTS, METHOD: In this study the feasibility of clot waveform analysis for monitoring of haemophilia A was investigated using blood samples from healthy controls as well as haemophilia Apatients under various clinical conditions. RESULTS:Thrombin, prothrombinase and tenase activity show a high correlation to factor VIII levels. Tenase activity was found to exhibit a linear relationship to factor VIII levels over a very large concentration range and was able to discriminate patients with severe, moderate and mild haemophilia. CONCLUSION: Clot waveform analysis is an easy, fast and cheap method to access disturbances in clot formation and can be done without any additional measurements beside an aPTT.
Authors: Cho Yeow Koh; Norrapat Shih; Christina Y C Yip; Aaron Wei Liang Li; Weiming Chen; Fathiah S Amran; Esther Jia En Leong; Janaki Krishnamoorthy Iyer; Grace Croft; Muhammad Ibrahim Bin Mazlan; Yen-Lin Chee; Eng-Soo Yap; Dougald M Monroe; Maureane Hoffman; Richard C Becker; Dominique P V de Kleijn; Vaishali Verma; Amita Gupta; Vijay K Chaudhary; A Mark Richards; R Manjunatha Kini; Mark Y Chan Journal: Nat Commun Date: 2021-11-25 Impact factor: 14.919
Authors: Saman Aghighi; Anne Riddell; Christine A Lee; Simon A Brown; Edward Tuddenham; Pratima Chowdary Journal: Res Pract Thromb Haemost Date: 2019-12-29