Hyun-Jin Tae1, Joon Ha Park2, Jeong-Hwi Cho2, In Hye Kim2, Ji Hyeon Ahn2, Jae Chul Lee2, Jong-Dai Kim3, Jinseu Park1, Soo Young Choi1, Moo-Ho Won4. 1. Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon 200-702, South Korea. 2. Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea. 3. Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, South Korea. 4. Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea. Email: mhwon@kangwon.ac.kr.
Abstract
BACKGROUND: Oenanthe javanica is an aquatic perennial herb originated from East Asia. Nowadays, the effects of Oenanthe javanica have been proven in various disease models. Studies regarding the antioxidant effect of Oenanthe javanica in the kidney are still unclear. METHODS: This study was therefore performed to investigate the effect of the Oenanthe javanica extract (OJE) in the rat kidney using immunohistochemistry for antioxidant enzymes, copper, zinc-superoxide dismutase (SOD1), manganese superoxide dismutase (SOD2), catalase (CAT) and glutathione peroxidase (GPx). Sprague-Dawley rats were randomly assigned to three groups: (1) normal diet fed-group (normal-group), (2) diet containing ascorbic acid (AA)-fed group (AA-group) as a positive control, (3) diet containing OJE-fed group (OJE-group). AA and OJE were supplied during 28 days. RESULTS: The side-effects were not observed in all the groups. Immunoreactivities of SOD1, SOD2, CAT and GPx were easily detected in the distal tubules of the kidney, and their immunoreactivities in the AA-and OJE-groups were increased to about 1.4-1.5 times and 2 times, respectively, compared with those in the normal-group. CONCLUSION: OJE significantly increased expressions of SOD1 & 2, CAT and GPx immunoreactivities in the distal tubules of the rat kidney, and this finding suggests that significant enhancements of endogenous enzymatic antioxidants by OJE treatment may be a legitimate strategy for decreasing oxidative stresses in the kidney.
BACKGROUND:Oenanthe javanica is an aquatic perennial herb originated from East Asia. Nowadays, the effects of Oenanthe javanica have been proven in various disease models. Studies regarding the antioxidant effect of Oenanthe javanica in the kidney are still unclear. METHODS: This study was therefore performed to investigate the effect of the Oenanthe javanica extract (OJE) in the rat kidney using immunohistochemistry for antioxidant enzymes, copper, zinc-superoxide dismutase (SOD1), manganese superoxide dismutase (SOD2), catalase (CAT) and glutathione peroxidase (GPx). Sprague-Dawley rats were randomly assigned to three groups: (1) normal diet fed-group (normal-group), (2) diet containing ascorbic acid (AA)-fed group (AA-group) as a positive control, (3) diet containing OJE-fed group (OJE-group). AA and OJE were supplied during 28 days. RESULTS: The side-effects were not observed in all the groups. Immunoreactivities of SOD1, SOD2, CAT and GPx were easily detected in the distal tubules of the kidney, and their immunoreactivities in the AA-and OJE-groups were increased to about 1.4-1.5 times and 2 times, respectively, compared with those in the normal-group. CONCLUSION: OJE significantly increased expressions of SOD1 & 2, CAT and GPx immunoreactivities in the distal tubules of the rat kidney, and this finding suggests that significant enhancements of endogenous enzymatic antioxidants by OJE treatment may be a legitimate strategy for decreasing oxidative stresses in the kidney.
Authors: Bai Hui Chen; Joon Ha Park; Jeong Hwi Cho; In Hye Kim; Bich Na Shin; Ji Hyeon Ahn; Seok Joon Hwang; Bing Chun Yan; Hyun Jin Tae; Jae Chul Lee; Eun Joo Bae; Yun Lyul Lee; Jong Dai Kim; Moo-Ho Won; Il Jun Kang Journal: Neural Regen Res Date: 2015-02 Impact factor: 5.135
Authors: Choong-Hyun Lee; Joon Ha Park; Ji Hyeon Ahn; Jong Dai Kim; Jun Hwi Cho; Tae-Kyeong Lee; Moo-Ho Won Journal: Iran J Basic Med Sci Date: 2019-08 Impact factor: 2.699