Xiaoyan Xu1, Jinhua Yang1, Ni Lin2, Guangying Yang1. 1. Department of Pathology, Zhengzhou People's Hospital, Southern Medical University, Zhengzhou 450000, China. 2. Huiqiao Division, Nanfang Hospital, Southern Medical University, Guangzhou 475000, China.
Abstract
OBJECTIVE: To evaluate the association of human papilloma virus (HPV) L1 capsid protein expression levels with cervical intraepithelial neoplasia (CIN) and its clinical significance. METHODS: Immunohistochemistry were performed to detect the expression of HPV L1 capsid protein in the cervical exfoliative cytological examination of 153 cases. The intensity (positive unit, PU) was assessed semi-quantitatively using ImagePro Plus image analysis software. The results were evaluated based on histopathologic diagnosis of cervical biopsy. RESULTS: PU of HPV L1 capsid protein in different cytopathological groups, including normal/inflammation, atypical squamous cells of unknown significance (ASCUS), low-grade squamous intraepithelial lesion (LSIL), high-grade squamous intraepithelial lesion (HSIL), was 46.87±24.46, 27.23±24.30, 24.10±22.45, 9.36±19.82, respectively, and the differences were statistically significant (P<0.01). PU of HPV L1 capsid protein in different histopathological groups, including normal cervixes or chronic cervicitis, LSIL and HSIL, was 41.30±26.66, 24.84±22.18, 8.69±19.20, respectively, and the differences were statistically significant (P<0.01). Patients with high-risk HPV16 and HPV18 had significantly lower PU of HPV L1 capsid protein than those with other high-risk HPV (P<0.01). PU of HPV L1 capsid protein were correlated negatively with both cytopathological groups and histopathological groups of cervical diseases (r=-0.458, P<0.01 and r=-0.441, P<0.01, respectively). PU of HPV L1 capsid protein was not associated with patients' age (P>0.05). CONCLUSION: Semi-quantitative analysis of HPV L1 capsid protein expression can directly reflect the precancerous progression of cervical cancer.
OBJECTIVE: To evaluate the association of human papilloma virus (HPV) L1 capsid protein expression levels with cervical intraepithelial neoplasia (CIN) and its clinical significance. METHODS: Immunohistochemistry were performed to detect the expression of HPV L1 capsid protein in the cervical exfoliative cytological examination of 153 cases. The intensity (positive unit, PU) was assessed semi-quantitatively using ImagePro Plus image analysis software. The results were evaluated based on histopathologic diagnosis of cervical biopsy. RESULTS: PU of HPV L1 capsid protein in different cytopathological groups, including normal/inflammation, atypical squamous cells of unknown significance (ASCUS), low-grade squamous intraepithelial lesion (LSIL), high-grade squamous intraepithelial lesion (HSIL), was 46.87±24.46, 27.23±24.30, 24.10±22.45, 9.36±19.82, respectively, and the differences were statistically significant (P<0.01). PU of HPV L1 capsid protein in different histopathological groups, including normal cervixes or chronic cervicitis, LSIL and HSIL, was 41.30±26.66, 24.84±22.18, 8.69±19.20, respectively, and the differences were statistically significant (P<0.01). Patients with high-risk HPV16 and HPV18 had significantly lower PU of HPV L1 capsid protein than those with other high-risk HPV (P<0.01). PU of HPV L1 capsid protein were correlated negatively with both cytopathological groups and histopathological groups of cervical diseases (r=-0.458, P<0.01 and r=-0.441, P<0.01, respectively). PU of HPV L1 capsid protein was not associated with patients' age (P>0.05). CONCLUSION: Semi-quantitative analysis of HPV L1 capsid protein expression can directly reflect the precancerous progression of cervical cancer.