Xinyuan Wang1, Ji Zhang2, Yao Cheng1, Jipei DU2, Degao Chen1, Zhimei Yang1, Yufang Wang1, Ning Huang1. 1. Department of Pathophysiology, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China. 2. Department of Forensic Genetics, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Abstract
OBJECTIVE: To detect the caspase-14 expression in malignant melanoma cells and tumor tissues and its effect on tumor resistance to drug. METHODS: The mRNA and protein level of caspase-14 in 4 melanoma cell lines (A375, A875, M14, and SK-Mel-1) and the melanocytes, was detected by reverse transcription PCR (RT-PCR) and Western blotting. Caspase-14 expression in 34 malignant melanoma tumor tissues and 10 dermal nevus tissues was determined by in situ hybridization and immunohistochemistry. RESULTS: Caspase-14 expression was seen in melanoma cells and melanocytes. It was higher in melanoma-associated antigen 1 recognized by T cells (MART-1) positive cells than in MART-1 negative cells. The cells expressing the lower caspase-14 were more sensitive to the treatment with either chemotherapy drugs camptothecin and cisplatin or radiotherapy than the ones expressing the higher caspase-14 (P<0.01). Caspase-14 expression was observed in 70% dermal nevus, as well as 97% in malignant melanoma tissues, and the difference between them was statistically significant (P<0.05). CONCLUSION: Caspase-14 is expressed in tissues and cells of malignant melanoma. Our data indicated that the expression level of caspase-14 affected the drug sensitivity of melanoma.
OBJECTIVE: To detect the caspase-14 expression in malignant melanoma cells and tumor tissues and its effect on tumor resistance to drug. METHODS: The mRNA and protein level of caspase-14 in 4 melanoma cell lines (A375, A875, M14, and SK-Mel-1) and the melanocytes, was detected by reverse transcription PCR (RT-PCR) and Western blotting. Caspase-14 expression in 34 malignant melanomatumor tissues and 10 dermal nevus tissues was determined by in situ hybridization and immunohistochemistry. RESULTS:Caspase-14 expression was seen in melanoma cells and melanocytes. It was higher in melanoma-associated antigen 1 recognized by T cells (MART-1) positive cells than in MART-1 negative cells. The cells expressing the lower caspase-14 were more sensitive to the treatment with either chemotherapy drugs camptothecin and cisplatin or radiotherapy than the ones expressing the higher caspase-14 (P<0.01). Caspase-14 expression was observed in 70% dermal nevus, as well as 97% in malignant melanoma tissues, and the difference between them was statistically significant (P<0.05). CONCLUSION:Caspase-14 is expressed in tissues and cells of malignant melanoma. Our data indicated that the expression level of caspase-14 affected the drug sensitivity of melanoma.