Challenges of genotyping polyploid species.

Most plant species are known to be either ancient or recent polyploids, containing more than one genome as a result of past interspecific hybridization events (allopolyploidy) and/or genome doubling (autopolyploidy). Genotyping in polyploid species offers a set of unique challenges. Most molecular marker methodologies are made more complex by polyploidy, as multilocus alleles are generally produced when a single locus is targeted. Genotyping by sequencing is also more challenging in polyploids, with problematic assemblies of duplicated regions and difficulties in distinguishing between inter- and intragenomic polymorphisms. Strategies for identifying and overcoming the challenges of polyploidy in plant genotyping are proposed.