Literature DB >> 25373753

Multiplexed digital gene expression analysis for genetical genomics in large plant populations.

Christian Obermeier1, Bertha M Salazar-Colqui, Viola Spamer, Rod Snowdon.   

Abstract

Digital gene expression (DGE) analysis is a cost-effective method for large-scale quantitative transcriptome analysis using second generation sequencing. Here we describe how adaptation of DGE with barcode indexing in large segregating plant populations of over 100 genotypes can be applied for successful expression QTL (eQTL) and gene expression network analysis to develop transcript-based markers for breeding.

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Year:  2015        PMID: 25373753     DOI: 10.1007/978-1-4939-1966-6_9

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  1 in total

1.  Seedling development traits in Brassica napus examined by gene expression analysis and association mapping.

Authors:  Niklas Körber; Anja Bus; Jinquan Li; Janet Higgins; Ian Bancroft; Erin Eileen Higgins; Isobel Alison Papworth Parkin; Bertha Salazar-Colqui; Rod John Snowdon; Benjamin Stich
Journal:  BMC Plant Biol       Date:  2015-06-09       Impact factor: 4.215

  1 in total

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