Literature DB >> 2537357

Monoclonal antibodies recognizing transforming growth factor-beta. Bioactivity neutralization and transforming growth factor beta 2 affinity purification.

J R Dasch1, D R Pace, W Waegell, D Inenaga, L Ellingsworth.   

Abstract

Four mAb able to recognize transforming growth factor-beta 2 (TGF-beta)2 were obtained. One of these mAb, 1D11.16, was able to neutralize the biological activity of both TGF-beta 1 and beta 2 in vitro. This was demonstrated in an Il-1, PHA-dependent thymocyte mitogenic assay that is inhibitable by TGF-beta in a dose-dependent manner. All four mAb recognized the dimeric form of TGF-beta 2 in Western blots. The mAb were also found to immunoprecipitate [125I]-TGF-beta 2. mAb 3C7.14 coupled to Sepharose could efficiently immunoaffinity purify TGF-beta 2 from a complex mixture of proteins. Affinity constants were determined for the four mAb and they ranged from 3.4 x 10(8) to 1.6 x 10(7) L/mol.

Entities:  

Mesh：

Substances：

Year:  1989        PMID： 2537357

Source DB:  PubMed          Journal:  J Immunol        ISSN： 0022-1767            Impact factor:   5.422


  112 in total

1.  Alveolar macrophages in neonatal mice are inherently unresponsive to Pneumocystis murina infection.

Authors:  Cathryn Kurkjian; Melissa Hollifield; J Louise Lines; Amy Rogosky; Kerry M Empey; Mahboob Qureshi; Stephen A Brown; Beth A Garvy
Journal:  Infect Immun       Date:  2012-06-04       Impact factor: 3.441

2.  Effector CD8 T cells possess suppressor function after 4-1BB and Toll-like receptor triggering.

Authors:  Lara Myers; Chikara Takahashi; Robert S Mittler; Robert J Rossi; Anthony T Vella
Journal:  Proc Natl Acad Sci U S A       Date:  2003-04-14       Impact factor: 11.205

3.  Affinity-based separations and purifications. Patents and literature.

Authors:  J S Dordick
Journal:  Appl Biochem Biotechnol       Date:  1991-01       Impact factor: 2.926

4.  Peptide ligands that use a novel binding site to target both TGF-β receptors.

Authors:  Lingyin Li; Brendan P Orner; Tao Huang; Andrew P Hinck; Laura L Kiessling
Journal:  Mol Biosyst       Date:  2010-10-04

5.  Development of tumor-infiltrating CD8+ T cell memory precursor effector cells and antimelanoma memory responses are the result of vaccination and TGF-β blockade during the perioperative period of tumor resection.

Authors:  Emily C Bellavance; Frederick J Kohlhapp; Andrew Zloza; Jeremy A O'Sullivan; James McCracken; Michael C Jagoda; Andrew T Lacek; Mitchell C Posner; Jose A Guevara-Patino
Journal:  J Immunol       Date:  2011-02-02       Impact factor: 5.422

6.  Renoprotective effects of anti-TGF-β antibody and antihypertensive therapies in Dahl S rats.

Authors:  Sydney R Murphy; Annette J Dahly-Vernon; Kathryn M J Dunn; Chun Cheng Andy Chen; Steven R Ledbetter; Jan M Williams; Richard J Roman
Journal:  Am J Physiol Regul Integr Comp Physiol       Date:  2012-04-25       Impact factor: 3.619

7.  CD69 downregulates autoimmune reactivity through active transforming growth factor-beta production in collagen-induced arthritis.

Authors:  David Sancho; Manuel Gómez; Fernando Viedma; Enric Esplugues; Mónica Gordón-Alonso; María Angeles García-López; Hortensia de la Fuente; Carlos Martínez-A; Pilar Lauzurica; Francisco Sánchez-Madrid
Journal:  J Clin Invest       Date:  2003-09       Impact factor: 14.808

8.  Transforming growth factor beta as a virulence mechanism for Leishmania braziliensis.

Authors:  A Barral; M Barral-Netto; E C Yong; C E Brownell; D R Twardzik; S G Reed
Journal:  Proc Natl Acad Sci U S A       Date:  1993-04-15       Impact factor: 11.205

9.  Elevated plasma transforming growth factor-beta 1 levels in breast cancer patients decrease after surgical removal of the tumor.

Authors:  F M Kong; M S Anscher; T Murase; B D Abbott; J D Iglehart; R L Jirtle
Journal:  Ann Surg       Date:  1995-08       Impact factor: 12.969

10.  Cell-cell signaling between adult rat ventricular myocytes and cardiac microvascular endothelial cells in heterotypic primary culture.

Authors:  M Nishida; J P Springhorn; R A Kelly; T W Smith
Journal:  J Clin Invest       Date:  1993-05       Impact factor: 14.808
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.