Literature DB >> 25372831

Crystallization and preliminary X-ray analysis of the hypothetical deaminase RPB_0146 from Rhodopseudomonas palustris HaA2.

Guofang Zhang1, Dan Yu1, Guodong Yang2, Hui Dong2, Tongcun Zhang1, Xiang Liu2.   

Abstract

RPB_0146, a putative deaminase from Rhodopseudomonas palustris HaA2, was expressed in Escherichia coli BL21 (DE3) cells and purified using a His6 tag by Ni2+-chelating affinity chromatography for X-ray crystallographic analysis. Diffraction-quality crystals were grown by the hanging-drop vapour-diffusion method at 289 K and diffracted to a resolution of 2.44 Å using a wavelength of 1.000 Å at the Photon Factory (KEK), Japan. The crystals belonged to the orthorhombic space group P2₁2₁2₁, with unit-cell parameters a=66.26, b=123.94, c=155.95 Å.

Entities:  

Keywords:  RPB_0146; Rhodopseudomonas palustris HaA2; deaminase

Mesh：

Substances：

Year:  2014        PMID： 25372831      PMCID： PMC4231866          DOI： 10.1107/S2053230X14018391

Source DB:  PubMed          Journal:  Acta Crystallogr F Struct Biol Commun        ISSN： 2053-230X            Impact factor:   1.056


  10 in total

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Journal:  J Mol Biol       Date:  1977-05-25       Impact factor: 5.469

4.  Solvent content of protein crystals.

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Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

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Review 6.  Degradation of chlorinated nitroaromatic compounds.

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7.  A novel 2-aminophenol 1,6-dioxygenase involved in the degradation of p-chloronitrobenzene by Comamonas strain CNB-1: purification, properties, genetic cloning and expression in Escherichia coli.

Authors:  Jian-Feng Wu; Cui-Wei Sun; Cheng-Ying Jiang; Zhi-Pei Liu; Shuang-Jiang Liu
Journal:  Arch Microbiol       Date:  2004-12-03       Impact factor: 2.552

8.  Anaerobic degradation of halogenated benzoic acids by photoheterotrophic bacteria.

Authors:  B J van der Woude; M de Boer; N M van der Put; F M van der Geld; R A Prins; J C Gottschal
Journal:  FEMS Microbiol Lett       Date:  1994-06-01       Impact factor: 2.742

9.  Novel partial reductive pathway for 4-chloronitrobenzene and nitrobenzene degradation in Comamonas sp. strain CNB-1.

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10.  Nucleotide sequence of plasmid pCNB1 from comamonas strain CNB-1 reveals novel genetic organization and evolution for 4-chloronitrobenzene degradation.

Authors:  Ying-Fei Ma; Jian-Feng Wu; Sheng-Yue Wang; Cheng-Ying Jiang; Yun Zhang; Su-Wei Qi; Lei Liu; Guo-Ping Zhao; Shuang-Jiang Liu
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  10 in total

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