| Literature DB >> 2537200 |
A J Garton1, D G Campbell, D Carling, D G Hardie, R J Colbran, S J Yeaman.
Abstract
Hormone-sensitive lipase is phosphorylated at a single site (site 2) in vitro by the AMP-activated protein kinase, without any direct effect on the activity of the enzyme. The amino acid sequence around this site has been determined. Ca2+/calmodulin-dependent protein kinase II also phosphorylates hormone-sensitive lipase predominantly at this site, whilst cyclic-GMP-dependent protein kinase phosphorylates exclusively the regulatory site (site 1) which is also phosphorylated by cyclic-AMP-dependent protein kinase. Phosphorylation of site 2 has been found to inhibit subsequent phosphorylation and activation of hormone-sensitive lipase by the cyclic-AMP-dependent and cyclic-GMP-dependent protein kinases, indicating that site-2 phosphorylation may have an antilipolytic role in vivo.Entities:
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Year: 1989 PMID： 2537200 DOI： 10.1111/j.1432-1033.1989.tb14548.x
Source DB: PubMed Journal: Eur J Biochem ISSN： 0014-2956