Romeo T Cristina1, Eugenia Dumitrescu1, Diana Brezovan2, Florin Muselin3, Viorica Chiurciu4. 1. Pharmacology and Pharmacy Dept., USAMVB, Faculty of Veterinary Medicine, 119, Calea Aradului, Timişoara, Romania. 2. Histology and Molecular biology Dept., USAMVB, Faculty of Veterinary Medicine, 119, Calea Aradului, Timişoara, Romania. 3. Botany Dept., USAMVB, Faculty of Veterinary Medicine, 119, Calea Aradului, Timişoara, Romania. 4. Drugs Production Dept., Romvac Company, Şos. Centurii no. 7, Voluntari, Romania.
Abstract
BACKGROUND: A pharmaco-chemical investigation of the Euphorbia cyparissias plant was justified by its known multiple therapeutic valences. Numerous components from extracts and latex of Euphorbiacae were identified, revealing a large plant family with a polyvalent therapeutic activity. MATERIALS AND METHODS: The aim of the study was to assess the skin tolerance level to irritation on different testing concentrations, of Euphorbia cyparissias extracts and ointments. Study was accomplished in rats and dogs, with the identification of all possible skin injuries and histological changes, after a simple patch test methodology. RESULTS: Ointment dermatological testing on rats, proved to be bearable on epilated skin at concentrations of 1, 2 and 5%. Ointments and mother tincture with higher concentrations (10% and 20%), led to irritation and cutis damages, and this was revealed through histology. CONCLUSION: Ointment tested on dog's skin was tolerable for epilated skin to concentrations of 1, 2 and/or 5%, additional testing on human volunteers confirmed the same situation.
BACKGROUND: A pharmaco-chemical investigation of the Euphorbia cyparissias plant was justified by its known multiple therapeutic valences. Numerous components from extracts and latex of Euphorbiacae were identified, revealing a large plant family with a polyvalent therapeutic activity. MATERIALS AND METHODS: The aim of the study was to assess the skin tolerance level to irritation on different testing concentrations, of Euphorbia cyparissias extracts and ointments. Study was accomplished in rats and dogs, with the identification of all possible skin injuries and histological changes, after a simple patch test methodology. RESULTS: Ointment dermatological testing on rats, proved to be bearable on epilated skin at concentrations of 1, 2 and 5%. Ointments and mother tincture with higher concentrations (10% and 20%), led to irritation and cutis damages, and this was revealed through histology. CONCLUSION: Ointment tested on dog's skin was tolerable for epilated skin to concentrations of 1, 2 and/or 5%, additional testing on human volunteers confirmed the same situation.
Entities:
Keywords:
Acanthosis; Euphorbia cyparissias; dermatology test; dog; ointments; rat