Camille Tagliaferri1,2,3, Jérôme Salles1,2, Jean-François Landrier4,5,6, Christophe Giraudet1,2, Véronique Patrac1,2, Patrice Lebecque1,2, Marie-Jeanne Davicco1,2, Audrey Chanet1,2, Corinne Pouyet1,2,7, Amélie Dhaussy3, Alain Huertas3, Yves Boirie1,2,8, Yohann Wittrant1,2, Véronique Coxam1,2, Stéphane Walrand9,10,11. 1. INRA, UMR 1019, UNH, CRNH Auvergne, 63000, Clermont-Ferrand, France. 2. Clermont Université, Université d'Auvergne, Unité de Nutrition Humaine, BP 10448, 63000, Clermont-Ferrand, France. 3. Lesieur, Asnieres-Sur-Seine, France. 4. UMR 1260, INRA, 13385, Marseille, France. 5. Nutrition, Obésité et Risque Thrombotique, UMR 1062, INSERM, 13385, Marseille, France. 6. Faculté de Médecine, Université d'Aix-Marseille, 13385, Marseille, France. 7. INRA, UMR 1019, Plateforme d'Exploration du Métabolisme, UNH, 63000, Clermont-Ferrand, France. 8. CHU Clermont-Ferrand, Service de Nutrition Clinique, 63003, Clermont-Ferrand, France. 9. INRA, UMR 1019, UNH, CRNH Auvergne, 63000, Clermont-Ferrand, France. swalrand@clermont.inra.fr. 10. Clermont Université, Université d'Auvergne, Unité de Nutrition Humaine, BP 10448, 63000, Clermont-Ferrand, France. swalrand@clermont.inra.fr. 11. Centre de recherche de Clermont-Theix-Lyon, 58 rue Montalembert, 63003, Clermont-Ferrand, France. swalrand@clermont.inra.fr.
Abstract
PURPOSE: The aim of this study was to evaluate and compare the musculoskeletal effects induced by ovariectomy-related fat mass deposition against the musculoskeletal effects caused by a high-fat diet. METHODS: A group of adult female rats was ovariectomized and fed a control diet. Two additional groups were sham-operated and fed a control or a high-fat diet for 19 weeks. Distal femur and serum bone parameters were measured to assess bone metabolism. Muscle protein metabolism, mitochondrial markers and triglyceride content were evaluated in tibialis anterior. Triglyceride content was evaluated in liver. Circulating inflammatory and metabolic markers were determined. RESULTS: The high-fat diet and ovariectomy led to similar increases in fat mass (+36.6-56.7%; p < 0.05) but had different impacts on bone and muscle tissues and inflammatory markers. Consumption of the high-fat diet led to decreased bone formation (-38.4%; p < 0.05), impaired muscle mitochondrial metabolism, muscle lipotoxicity and a 20.9% increase in tibialis anterior protein synthesis rate (p < 0.05). Ovariectomy was associated with higher bone turnover as bone formation increased +72.7% (p < 0.05) and bone resorption increased +76.4% (p < 0.05), leading to bone loss, a 17.9% decrease in muscle protein synthesis rate (p < 0.05) and liver lipotoxicity. CONCLUSIONS: In female rats, high-fat diet and ovariectomy triggered similar gains in fat mass but had different impacts on bone and muscle metabolism. The ovariectomy-induced mechanisms affecting the musculoskeletal system are mainly caused by estrogen depletion, which surpasses the potential-independent effect of adiposity.
PURPOSE: The aim of this study was to evaluate and compare the musculoskeletal effects induced by ovariectomy-related fat mass deposition against the musculoskeletal effects caused by a high-fat diet. METHODS: A group of adult female rats was ovariectomized and fed a control diet. Two additional groups were sham-operated and fed a control or a high-fat diet for 19 weeks. Distal femur and serum bone parameters were measured to assess bone metabolism. Muscle protein metabolism, mitochondrial markers and triglyceride content were evaluated in tibialis anterior. Triglyceride content was evaluated in liver. Circulating inflammatory and metabolic markers were determined. RESULTS: The high-fat diet and ovariectomy led to similar increases in fat mass (+36.6-56.7%; p < 0.05) but had different impacts on bone and muscle tissues and inflammatory markers. Consumption of the high-fat diet led to decreased bone formation (-38.4%; p < 0.05), impaired muscle mitochondrial metabolism, muscle lipotoxicity and a 20.9% increase in tibialis anterior protein synthesis rate (p < 0.05). Ovariectomy was associated with higher bone turnover as bone formation increased +72.7% (p < 0.05) and bone resorption increased +76.4% (p < 0.05), leading to bone loss, a 17.9% decrease in muscle protein synthesis rate (p < 0.05) and liver lipotoxicity. CONCLUSIONS: In female rats, high-fat diet and ovariectomy triggered similar gains in fat mass but had different impacts on bone and muscle metabolism. The ovariectomy-induced mechanisms affecting the musculoskeletal system are mainly caused by estrogen depletion, which surpasses the potential-independent effect of adiposity.
Authors: Eija Pöllänen; Paula H A Ronkainen; Mia Horttanainen; Timo Takala; Jukka Puolakka; Harri Suominen; Sarianna Sipilä; Vuokko Kovanen Journal: Growth Horm IGF Res Date: 2010-08-17 Impact factor: 2.372
Authors: Sarah M Greising; Kristen A Baltgalvis; Dawn A Lowe; Gordon L Warren Journal: J Gerontol A Biol Sci Med Sci Date: 2009-06-26 Impact factor: 6.053
Authors: Marta G Novelle; María J Vázquez; Juan R Peinado; Kátia D Martinello; Miguel López; Simon M Luckman; Manuel Tena-Sempere; María M Malagón; Rubén Nogueiras; Carlos Diéguez Journal: Sci Rep Date: 2017-04-07 Impact factor: 4.379