BACKGROUND: Enterocolitis (EC) is the most common and serious postoperative complication of Hirschsprung's disease (HD). Probiotics potentially play a protective role in maintaining intestinal mucosal integrity. Based on the beneficial effects of probiotics, we hypothesized that oral probiotics could decrease the incidence and severity of Hirschsprung's disease-associated enterocolitis (HAEC). METHODS: We conducted a prospective, multicenter, randomized, and controlled trial to assess whether oral probiotics could decrease the incidence and severity of HAEC. HD patients were randomly assigned into the control group and probiotic-treated group. All children in probiotic-treated group were fed with probiotics per day for 4 weeks. In next 3 months, the incidence and severity of HAEC were analyzed. The peripheral blood T lymphocyte subsets and cytokines, including TNF-α, IFN-γ, IL-6, and IL-10, were analyzed by flow cytometry and enzyme immunoassay (EIA). RESULTS: Compared with the control group, the incidence of HAEC in the probiotic-treated group was significantly diminished. The severity of EC was also remarkably decreased. Furthermore, probiotics balanced T lymphocyte subsets. Moreover, pro-inflammatory cytokinesTNF-α, IFN-γ, and IL-6 were significantly decreased and anti-inflammatory cytokine IL-10 was notably increased in probiotic-treated group. CONCLUSIONS:Probiotics not only significantly diminished the incidence but also decreased the severity of HAEC. Moreover, our study revealed that probiotics decreased pro-inflammatory cytokine and increased anti-inflammatory cytokine and furthermore balanced T lymphocytes (registered with ClinicalTrials.gov, NCT number: NCT01934959).
RCT Entities:
BACKGROUND:Enterocolitis (EC) is the most common and serious postoperative complication of Hirschsprung's disease (HD). Probiotics potentially play a protective role in maintaining intestinal mucosal integrity. Based on the beneficial effects of probiotics, we hypothesized that oral probiotics could decrease the incidence and severity of Hirschsprung's disease-associated enterocolitis (HAEC). METHODS: We conducted a prospective, multicenter, randomized, and controlled trial to assess whether oral probiotics could decrease the incidence and severity of HAEC. HDpatients were randomly assigned into the control group and probiotic-treated group. All children in probiotic-treated group were fed with probiotics per day for 4 weeks. In next 3 months, the incidence and severity of HAEC were analyzed. The peripheral blood T lymphocyte subsets and cytokines, including TNF-α, IFN-γ, IL-6, and IL-10, were analyzed by flow cytometry and enzyme immunoassay (EIA). RESULTS: Compared with the control group, the incidence of HAEC in the probiotic-treated group was significantly diminished. The severity of EC was also remarkably decreased. Furthermore, probiotics balanced T lymphocyte subsets. Moreover, pro-inflammatory cytokines TNF-α, IFN-γ, and IL-6 were significantly decreased and anti-inflammatory cytokine IL-10 was notably increased in probiotic-treated group. CONCLUSIONS: Probiotics not only significantly diminished the incidence but also decreased the severity of HAEC. Moreover, our study revealed that probiotics decreased pro-inflammatory cytokine and increased anti-inflammatory cytokine and furthermore balanced T lymphocytes (registered with ClinicalTrials.gov, NCT number: NCT01934959).
Authors: D H Teitelbaum; R E Cilley; N J Sherman; D Bliss; N D Uitvlugt; E J Renaud; I Kirstioglu; T Bengston; A G Coran Journal: Ann Surg Date: 2000-09 Impact factor: 12.969
Authors: Andrew R Barclay; Ben Stenson; Judith H Simpson; Lawrence T Weaver; David C Wilson Journal: J Pediatr Gastroenterol Nutr Date: 2007-11 Impact factor: 2.839
Authors: Eliana A G Reis; José E Hagan; Guilherme S Ribeiro; Andrea Teixeira-Carvalho; Olindo A Martins-Filho; Ruth R Montgomery; Albert C Shaw; Albert I Ko; Mitermayer G Reis Journal: PLoS Negl Trop Dis Date: 2013-09-19
Authors: Ankush Gosain; Philip K Frykman; Robert A Cowles; John Horton; Marc Levitt; David H Rothstein; Jacob C Langer; Allan M Goldstein Journal: Pediatr Surg Int Date: 2017-02-02 Impact factor: 1.827
Authors: Weibing Tang; Yang Su; Chen Yuan; Yuqing Zhang; Lingling Zhou; Lei Peng; Pin Wang; Guanglin Chen; Yang Li; Hongxing Li; Zhengke Zhi; Hang Chang; Bo Hang; Jian-Hua Mao; Antoine M Snijders; Yankai Xia Journal: Gut Microbes Date: 2020-01-16
Authors: Akemi L Kawaguchi; Yigit S Guner; Stig Sømme; Alexandria C Quesenberry; L Grier Arthur; Juan E Sola; Cynthia D Downard; Rebecca M Rentea; Patricia A Valusek; Caitlin A Smith; Mark B Slidell; Robert L Ricca; Roshni Dasgupta; Elizabeth Renaud; Doug Miniati; Jarod McAteer; Alana L Beres; Julia Grabowski; Shawn D St Peter; Ankush Gosain Journal: J Pediatr Surg Date: 2021-03-28 Impact factor: 2.549