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Literature DB >> 2536990

Recovery of structurally intact and infectious poliovirus type 1 from HeLa cells during receptor-mediated endocytosis.

P Willingmann¹, H Barnert, H Zeichhardt, K O Habermehl.

Abstract

Poliovirus type 1 enters HeLa cells by receptor-mediated endocytosis as an intact virus. Up to 30 min after adsorption complete virus particles still containing VP4 and sedimenting with 156 S could be recovered from the cells. These virus particles were N-antigenic and infectious. Thirty minutes after adsorption the recovery of intact and infectious virus decreased. This decrease presumably reflects viral uncoating in the acidic endosomes and/or lysosomes because virus particles could be localized in endosomes at this time. The direct involvement of clathrin-coated structures in the endocytosis of poliovirus has been deduced from the enclosure of poliovirus in coated vesicles at 10 min after adsorption. At this time intact and infectious virus could be recovered only after the coated vesicles were disrupted by treatment with 0.5 M Tris at pH 7.0.

Entities: Chemical Species

Mesh：

Substances：
Receptors, Virus

Year: 1989 PMID： 2536990 DOI： 10.1016/0042-6822(89)90286-9

Source DB: PubMed Journal: Virology ISSN： 0042-6822 Impact factor: 3.616

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Cited

15 in total

1. Molecular tectonic model of virus structural transitions: the putative cell entry states of poliovirus.

Authors: D M Belnap; D J Filman; B L Trus; N Cheng; F P Booy; J F Conway; S Curry; C N Hiremath; S K Tsang; A C Steven; J M Hogle
Journal: J Virol Date: 2000-02 Impact factor: 5.103

2. Functional domains of the poliovirus receptor.

Authors: S Koike; I Ise; A Nomoto
Journal: Proc Natl Acad Sci U S A Date: 1991-05-15 Impact factor: 11.205

3. Lack of myristoylation of poliovirus capsid polypeptide VP0 prevents the formation of virions or results in the assembly of noninfectious virus particles.

Authors: D Marc; G Masson; M Girard; S van der Werf
Journal: J Virol Date: 1990-09 Impact factor: 5.103

Recovery of structurally intact and infectious poliovirus type 1 from HeLa cells during receptor-mediated endocytosis.

1. Molecular tectonic model of virus structural transitions: the putative cell entry states of poliovirus.

2. Functional domains of the poliovirus receptor.

3. Lack of myristoylation of poliovirus capsid polypeptide VP0 prevents the formation of virions or results in the assembly of noninfectious virus particles.

4. Kinetics of poliovirus uncoating in HeLa cells in a nonacidic environment.

5. Cold-adapted poliovirus mutants bypass a postentry replication block.

6. Uncoating of human rhinovirus serotype 2 from late endosomes.

7. Internalization of echovirus 1 in caveolae.

8. The clathrin endocytic pathway in viral infection.

9. Receptor (CD155)-dependent endocytosis of poliovirus and retrograde axonal transport of the endosome.

10. The Synthetic Antiviral Drug Arbidol Inhibits Globally Prevalent Pathogenic Viruses.