Literature DB >> 2536910

Clinical evaluation of a new herpes simplex virus ELISA: a rapid diagnostic test for herpes simplex virus.

D A Baker1, B Gonik, P O Milch, A Berkowitz, S Lipson, U Verma.   

Abstract

A new sensitive and rapid enzyme-linked immunosorbent assay (ELISA) test (Du Pont HERPCHEK) for the detection of herpes simplex virus (HSV) antigen was evaluated. A total of 563 clinical samples collected from patients attending obstetrics and gynecology and sexually transmitted disease clinics in two geographic locations were tested by ELISA, and the results were compared with virus isolation in cell culture. The sensitivity and specificity of the ELISA were 97.5 and 98.6%, respectively. When a confirmatory blocking ELISA was used to demonstrate the presence of HSV antigen in culture-negative but ELISA-positive samples, the specificity of the assay increased to 100%. The assay is easy to perform and may be used to diagnose HSV infection rapidly without the need for culture confirmation.

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Year:  1989        PMID： 2536910

Source DB:  PubMed          Journal:  Obstet Gynecol        ISSN： 0029-7844            Impact factor:   7.661


  10 in total

1.  Evaluation of an automated immunodiagnostic assay system for direct detection of herpes simplex virus antigen in clinical specimens.

Authors:  S L Johnston; S Hamilton; R Bindra; D A Hursh; C A Gleaves
Journal:  J Clin Microbiol       Date:  1992-04       Impact factor: 5.948

2.  Comparison of standard culture methods, a shell vial assay, and a DNA probe for the detection of herpes simplex virus.

Authors:  L A Seal; P S Toyama; K M Fleet; K S Lerud; S R Heth; A J Moorman; J C Woods; R B Hill
Journal:  J Clin Microbiol       Date:  1991-03       Impact factor: 5.948

3.  Comparison of two enzyme-linked immunosorbent assays for detection of herpes simplex virus antigen.

Authors:  B Gonik; M Seibel; A Berkowitz; M B Woodin; K Mills
Journal:  J Clin Microbiol       Date:  1991-03       Impact factor: 5.948

4.  The laboratory diagnosis of herpes simplex virus infections.

Authors:  Ameeta Singh; Jutta Preiksaitis; Alex Ferenczy; Barbara Romanowski
Journal:  Can J Infect Dis Med Microbiol       Date:  2005-03       Impact factor: 2.471

5.  Comparison of three monoclonal antibody-based enzyme immunoassays for detection of herpes simplex virus in clinical specimens.

Authors:  J P Brinker; J E Herrmann
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1995-04       Impact factor: 3.267

Review 6.  Laboratory techniques in the diagnosis of herpes simplex infection.

Authors:  R L Ashley
Journal:  Genitourin Med       Date:  1993-06

7.  Evaluation of new transport medium for detection of herpes simplex virus by culture and direct enzyme-linked immunosorbent assay.

Authors:  J R Ogburn; J T Hoffpauir; E Cole; K Hood; D Michael; T Nguyen; S Raden; B Raju; V Reisinger; P E Oefinger
Journal:  J Clin Microbiol       Date:  1994-12       Impact factor: 5.948

8.  Herpes simplex virus antigen direct detection in standard virus transport medium by Du Pont Herpchek enzyme-linked immunosorbent assay.

Authors:  L Verano; F J Michalski
Journal:  J Clin Microbiol       Date:  1990-11       Impact factor: 5.948

9.  Sandwich enzyme immunoassay and latex agglutination test for herpes simplex virus keratitis.

Authors:  S F Lee; J S Pepose
Journal:  J Clin Microbiol       Date:  1990-04       Impact factor: 5.948

10.  A Novel Method to Titrate Herpes Simplex Virus-1 (HSV-1) Using Laser-Based Scanning of Near-Infrared Fluorophores Conjugated Antibodies.

Authors:  Marco Fabiani; Dolores Limongi; Anna Teresa Palamara; Giovanna De Chiara; Maria Elena Marcocci
Journal:  Front Microbiol       Date:  2017-06-14       Impact factor: 5.640
  10 in total

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