Stephanie H Lim1, Wei Chua2, Christina Cheng3, Joseph Descallar4, Weng Ng2, Michael Solomon5, Les Bokey6, Karen Wong7, Mark T Lee8, Paul de Souza9, Joo-Shik Shin10, Cheok Soon Lee11. 1. Department of Medical Oncology, Liverpool Hospital, Liverpool, Australia Ingham Institute for Applied Medical Research, Liverpool, Australia University of New South Wales, Kensington, Australia stephanie.lim@sswahs.nsw.gov.au. 2. Department of Medical Oncology, Liverpool Hospital, Liverpool, Australia Ingham Institute for Applied Medical Research, Liverpool, Australia. 3. Molecular Medicine Research Group, University of Western Sydney, Liverpool, Australia. 4. Ingham Institute for Applied Medical Research, Liverpool, Australia University of New South Wales, Kensington, Australia. 5. Department of Colorectal Surgery, Royal Prince Alfred Hospital, Camperdown, Australia University of Sydney, Camperdown, Australia Surgical Outcomes Research Centre, Sydney, Australia. 6. Ingham Institute for Applied Medical Research, Liverpool, Australia University of Western Sydney, Campbelltown, Australia Department of Colorectal Surgery, Liverpool Hospital, Liverpool, Australia. 7. Ingham Institute for Applied Medical Research, Liverpool, Australia University of New South Wales, Kensington, Australia Department of Radiation Oncology, Liverpool Hospital, Liverpool, Australia. 8. University of New South Wales, Kensington, Australia Department of Radiation Oncology, Liverpool Hospital, Liverpool, Australia. 9. Department of Medical Oncology, Liverpool Hospital, Liverpool, Australia Ingham Institute for Applied Medical Research, Liverpool, Australia University of New South Wales, Kensington, Australia Molecular Medicine Research Group, University of Western Sydney, Liverpool, Australia University of Western Sydney, Campbelltown, Australia. 10. Ingham Institute for Applied Medical Research, Liverpool, Australia Molecular Medicine Research Group, University of Western Sydney, Liverpool, Australia University of Western Sydney, Campbelltown, Australia Department of Anatomical Pathology, Liverpool Hospital, Liverpool, Australia. 11. Ingham Institute for Applied Medical Research, Liverpool, Australia University of New South Wales, Kensington, Australia Molecular Medicine Research Group, University of Western Sydney, Liverpool, Australia University of Sydney, Camperdown, Australia University of Western Sydney, Campbelltown, Australia Department of Anatomical Pathology, Liverpool Hospital, Liverpool, Australia.
Abstract
BACKGROUND:Lymphocytes and natural killer cells (NK) appear to be important in colorectal cancer. Their role in chemoradiotherapy for rectal cancers is unclear. We evaluated T-lymphocytes (CD3), sub-groups CD4 and CD8, and NK cells (CD56+CD57) in normal and rectal tumor tissues pre- and post-chemoradiotherapy, and investigated their relationship to tumor regression grade, disease-free survival and pathological stage. MATERIALS AND METHODS: Tissue microarrays from colonoscopic biopsies, resection specimens and normal tissues, from 52 patients, were immunostained. RESULTS:NK cell counts were significantly lower in tumor samples compared to normal tissues (p=0.007). T-lymphocyte counts were higher in post-treatment compared to pre-treatment samples (p=0.025), specifically in the CD8 subgroup after long-course treatment. The results suggested an association between post-treatment CD8 and NK cell counts with higher tumor regression. No associations were found with regard to stage or disease-free survival. CONCLUSION:NK cell counts were significantly reduced in rectal cancers compared to normal tissues, while total T-lymphocyte counts increased post-chemoradiotherapy. Both appeared important in tumor regression. Copyright
RCT Entities:
BACKGROUND: Lymphocytes and natural killer cells (NK) appear to be important in colorectal cancer. Their role in chemoradiotherapy for rectal cancers is unclear. We evaluated T-lymphocytes (CD3), sub-groups CD4 and CD8, and NK cells (CD56+CD57) in normal and rectal tumor tissues pre- and post-chemoradiotherapy, and investigated their relationship to tumor regression grade, disease-free survival and pathological stage. MATERIALS AND METHODS: Tissue microarrays from colonoscopic biopsies, resection specimens and normal tissues, from 52 patients, were immunostained. RESULTS: NK cell counts were significantly lower in tumor samples compared to normal tissues (p=0.007). T-lymphocyte counts were higher in post-treatment compared to pre-treatment samples (p=0.025), specifically in the CD8 subgroup after long-course treatment. The results suggested an association between post-treatment CD8 and NK cell counts with higher tumor regression. No associations were found with regard to stage or disease-free survival. CONCLUSION: NK cell counts were significantly reduced in rectal cancers compared to normal tissues, while total T-lymphocyte counts increased post-chemoradiotherapy. Both appeared important in tumor regression. Copyright
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