Romina F Aromando1, Ana R Raimondi2, Miguel A Pérez3, Veronica A Trivillin4, Amanda E Schwint4, Maria E Itoiz3. 1. Department of Oral Pathology, Faculty of Dentistry, University of Buenos Aires, Buenos Aires, Argentina romina_aromando@yahoo.com.ar. 2. Research Area Institute of Oncology Angel H. Roffo, School of Medicine, University of Buenos Aires, Buenos Aires, Argentina. 3. Department of Oral Pathology, Faculty of Dentistry, University of Buenos Aires, Buenos Aires, Argentina. 4. Department of Radiobiology, National Atomic Energy Commission, Buenos Aires, Argentina.
Abstract
AIM: To evaluate vascular morphology and density, angiogenic switch activation, vascular endothelial growth factor (VEGF) expression, and endothelial cell (EC) proliferation in the hamster cheek pouch (HCP) model of oral cancer. MATERIALS AND METHODS: Immunohistochemical detection of factor VIII, 5'-Bromo-2'-Deoxyuridine (BrdU) and VEGF was performed in pre-malignant and tumoral tissues. RESULTS: Activation of angiogenesis was detected adjacent to epithelial dysplasia. Vascularized area and perimeter (p<0.001) increased in dysplasias and tumors. Tumor blood vessels exhibited an enhanced vascular compression (p<0.001) and structural alterations. EC proliferation was similar in dysplasias and carcinomas. An increase in vascular density, EC proliferation and VEGF expression was found in potentially malignant tissues but not in carcinomas. CONCLUSION: The angiogenic switch occurs in the dysplastic stage preceding tumor development in the HCP model of oral cancer. In potentially malignant tissues, increased VEGF expression favors EC proliferation and an increase in vascular density. Conversely, in tumors, VEGF is no longer of pivotal importance. Copyright
AIM: To evaluate vascular morphology and density, angiogenic switch activation, vascular endothelial growth factor (VEGF) expression, and endothelial cell (EC) proliferation in the hamster cheek pouch (HCP) model of oral cancer. MATERIALS AND METHODS: Immunohistochemical detection of factor VIII, 5'-Bromo-2'-Deoxyuridine (BrdU) and VEGF was performed in pre-malignant and tumoral tissues. RESULTS: Activation of angiogenesis was detected adjacent to epithelial dysplasia. Vascularized area and perimeter (p<0.001) increased in dysplasias and tumors. Tumor blood vessels exhibited an enhanced vascular compression (p<0.001) and structural alterations. EC proliferation was similar in dysplasias and carcinomas. An increase in vascular density, EC proliferation and VEGF expression was found in potentially malignant tissues but not in carcinomas. CONCLUSION: The angiogenic switch occurs in the dysplastic stage preceding tumor development in the HCP model of oral cancer. In potentially malignant tissues, increased VEGF expression favors EC proliferation and an increase in vascular density. Conversely, in tumors, VEGF is no longer of pivotal importance. Copyright
Authors: Andrea Monti Hughes; Jessica A Goldfinger; Mónica A Palmieri; Paula Ramos; Iara S Santa Cruz; Luciana De Leo; Marcela A Garabalino; Silvia I Thorp; Paula Curotto; Emiliano C C Pozzi; Kazuki Kawai; Shinichi Sato; María E Itoiz; Verónica A Trivillin; Juan S Guidobono; Hiroyuki Nakamura; Amanda E Schwint Journal: Life (Basel) Date: 2022-07-20