STUDY OBJECTIVES: Although obstructive sleep apnea (OSA) causes cardiovascular morbidities through atherosclerosis induced by inflammation and endothelial dysfunction, OSA patients exhibit elevated plasma vascular endothelial growth factor (VEGF), which may represent an adaptive response to intermittent hypoxia. The aims of this study were to investigate whether in vitro endothelial wound healing and monocyte migration are affected by patient serum, and to determine the implication of circulating factors (VEGF and C-reactive protein). PATIENTS: Serum was collected from healthy controls (HC), "healthy" OSA, and metabolic syndrome (MS) patients with or without OSA. MEASUREMENTS AND RESULTS: Along with the presence of OSA and/or MS, both VEGF and hsCRP were significantly elevated in patient serum. Their specific role was tested with blocking antibodies on primary endothelial cells for wound healing assay and on human monocytes for migration assay. Endothelial wound healing was reduced with OSA compared to HC serum, and even more significantly using MS+OSA patient serum. Altered wound healing with OSA serum was unmasked when blocking VEGF and restored when blocking CRP. Monocyte migration was activated with OSA serum, and further enhanced by MS+OSA patient serum. Blocking CRP in serum inhibited this migration. CONCLUSIONS: Serum from OSA patient alters in vitro endothelial cell repair function and activates monocyte migration; this is further aggravated with the presence of metabolic syndrome. These effects are partly driven by VEGF and CRP, suggesting an unfavorable balance between the pro healing (VEGF) and pro injury (CRP) factors that may promote vascular injury in OSA with and without metabolic syndrome.
STUDY OBJECTIVES: Although obstructive sleep apnea (OSA) causes cardiovascular morbidities through atherosclerosis induced by inflammation and endothelial dysfunction, OSA patients exhibit elevated plasma vascular endothelial growth factor (VEGF), which may represent an adaptive response to intermittent hypoxia. The aims of this study were to investigate whether in vitro endothelial wound healing and monocyte migration are affected by patient serum, and to determine the implication of circulating factors (VEGF and C-reactive protein). PATIENTS: Serum was collected from healthy controls (HC), "healthy" OSA, and metabolic syndrome (MS) patients with or without OSA. MEASUREMENTS AND RESULTS: Along with the presence of OSA and/or MS, both VEGF and hsCRP were significantly elevated in patient serum. Their specific role was tested with blocking antibodies on primary endothelial cells for wound healing assay and on human monocytes for migration assay. Endothelial wound healing was reduced with OSA compared to HC serum, and even more significantly using MS+OSA patient serum. Altered wound healing with OSA serum was unmasked when blocking VEGF and restored when blocking CRP. Monocyte migration was activated with OSA serum, and further enhanced by MS+OSA patient serum. Blocking CRP in serum inhibited this migration. CONCLUSIONS: Serum from OSA patient alters in vitro endothelial cell repair function and activates monocyte migration; this is further aggravated with the presence of metabolic syndrome. These effects are partly driven by VEGF and CRP, suggesting an unfavorable balance between the pro healing (VEGF) and pro injury (CRP) factors that may promote vascular injury in OSA with and without metabolic syndrome.
Authors: Mette K Hagensen; Merete K Raarup; Martin B Mortensen; Troels Thim; Jens R Nyengaard; Erling Falk; Jacob F Bentzon Journal: Cardiovasc Res Date: 2011-10-19 Impact factor: 10.787
Authors: Sanja Jelic; Margherita Padeletti; Steven M Kawut; Christopher Higgins; Stephen M Canfield; Duygu Onat; Paolo C Colombo; Robert C Basner; Phillip Factor; Thierry H LeJemtel Journal: Circulation Date: 2008-04-14 Impact factor: 29.690
Authors: Maurice Dematteis; Cécile Julien; Christiane Guillermet; Nathalie Sturm; Sylvie Lantuejoul; Michel Mallaret; Patrick Lévy; Evelyne Gozal Journal: Am J Respir Crit Care Med Date: 2007-10-25 Impact factor: 21.405
Authors: Kim Van der Heiden; Frank J H Gijsen; Andrew Narracott; Sarah Hsiao; Ian Halliday; Julian Gunn; Jolanda J Wentzel; Paul C Evans Journal: Cardiovasc Res Date: 2013-04-15 Impact factor: 10.787
Authors: Isaac Almendros; Miguel Ángel Martínez-García; Francisco Campos-Rodríguez; Erica Riveiro-Falkenbach; José L Rodríguez-Peralto; Eduardo Nagore; Antonio Martorell-Calatayud; Luis Hernández Blasco; Jose Bañuls Roca; Eusebi Chiner Vives; Alicia Sánchez-de-la-Torre; Jorge Abad-Capa; Josep Maria Montserrat; Amalia Pérez-Gil; Valentín Cabriada-Nuño; Irene Cano-Pumarega; Jaime Corral-Peñafiel; Trinidad Diaz-Cambriles; Olga Mediano; Joan Dalmau-Arias; Ramon Farré; David Gozal Journal: Front Neurol Date: 2018-04-26 Impact factor: 4.003