BACKGROUND: (68)Ga-triacetylfusarinine C (TAFC) and (68)Ga-ferrioxamine E (FOXE) show great potential to be used as highly sensitive and selective tracers for Aspergillus infection imaging. Here we report on a comparison of the ex vivo biodistribution and small animal imaging of (68)Ga-TAFC and (68)Ga-FOXE versus (68)Ga-colloid and (68)Ga-citrate as unspecific control in mice. METHODS: The radiochemical purity of tested (68)Ga labelled tracers was determined by RP-HPLC or ITLC-SG. Ex vivo biodistribution was studied in normal DBA/2 mice 30 min and 90 min p.i. Static and dynamic imaging were performed using µPET/CT. RESULTS: (68)Ga-TAFC and (68)Ga-FOXE showed rapid renal excretion and low blood values even 90 min p.i. (68)Ga-TAFC showed almost no retention in other organs while (68)Ga-FOXE displayed some uptake in gastrointestinal tract. (68)Ga-colloid and (68)Ga-citrate revealed significantly different ex vivo biodistribution. (68)Ga-colloid showed pronounced radioactivity retention in the liver, while (68)Ga-citrate displayed high blood values and significant retention of radioactivity in highly perfused organs. CONCLUSIONS: From the results, both (68)Ga-TAFC and (68)Ga-FOXE have excellent and significantly different in vivo behaviour compared to (68)Ga-colloid and (68)Ga-citrate. (68)Ga-TAFC in particular confirmed its great potential use as a specific tracer for Aspergillus infection imaging.
BACKGROUND: (68)Ga-triacetylfusarinine C (TAFC) and (68)Ga-ferrioxamine E (FOXE) show great potential to be used as highly sensitive and selective tracers for Aspergillus infection imaging. Here we report on a comparison of the ex vivo biodistribution and small animal imaging of (68)Ga-TAFC and (68)Ga-FOXE versus (68)Ga-colloid and (68)Ga-citrate as unspecific control in mice. METHODS: The radiochemical purity of tested (68)Ga labelled tracers was determined by RP-HPLC or ITLC-SG. Ex vivo biodistribution was studied in normal DBA/2 mice 30 min and 90 min p.i. Static and dynamic imaging were performed using µPET/CT. RESULTS: (68)Ga-TAFC and (68)Ga-FOXE showed rapid renal excretion and low blood values even 90 min p.i. (68)Ga-TAFC showed almost no retention in other organs while (68)Ga-FOXE displayed some uptake in gastrointestinal tract. (68)Ga-colloid and (68)Ga-citrate revealed significantly different ex vivo biodistribution. (68)Ga-colloid showed pronounced radioactivity retention in the liver, while (68)Ga-citrate displayed high blood values and significant retention of radioactivity in highly perfused organs. CONCLUSIONS: From the results, both (68)Ga-TAFC and (68)Ga-FOXE have excellent and significantly different in vivo behaviour compared to (68)Ga-colloid and (68)Ga-citrate. (68)Ga-TAFC in particular confirmed its great potential use as a specific tracer for Aspergillus infection imaging.
Authors: John W Baddley; David R Andes; Kieren A Marr; Dimitrios P Kontoyiannis; Barbara D Alexander; Carol A Kauffman; Robert A Oster; Elias J Anaissie; Thomas J Walsh; Mindy G Schuster; John R Wingard; Thomas F Patterson; James I Ito; O Dale Williams; Tom Chiller; Peter G Pappas Journal: Clin Infect Dis Date: 2010-06-15 Impact factor: 9.079
Authors: F Sánchez; A Orero; A Soriano; C Correcher; P Conde; A González; L Hernández; L Moliner; M J Rodríguez-Alvarez; L F Vidal; J M Benlloch; S E Chapman; W M Leevy Journal: Med Phys Date: 2013-05 Impact factor: 4.071
Authors: Johanna Mu Silvola; Iina Laitinen; Henri J Sipilä; V Jukka O Laine; Pia Leppänen; Seppo Ylä-Herttuala; Juhani Knuuti; Anne Roivainen Journal: EJNMMI Res Date: 2011-08-17 Impact factor: 3.138
Authors: George Firth; Julia E Blower; Joanna J Bartnicka; Aishwarya Mishra; Aidan M Michaels; Alex Rigby; Afnan Darwesh; Fahad Al-Salemee; Philip J Blower Journal: RSC Chem Biol Date: 2022-04-11