Literature DB >> 2536089

Generation of a helper cell line for packaging avian leukosis virus-based vectors.

P Savatier1, C Bagnis, P Thoraval, D Poncet, M Belakebi, F Mallet, C Legras, F L Cosset, J L Thomas, Y Chebloune.   

Abstract

We constructed an avian leukosis virus-based packaging cell line, pHF-g, containing Rous-associated virus DNA with several alterations to abolish RNA packaging. One of them is a 52-base-pair deletion encompassing the putative encapsidation signal in the leader region. The 3' long terminal repeat was also removed and replaced by the polyadenylation sequence from the herpes simplex virus thymidine kinase gene. When pHF-g cells were transfected by an avian leukosis virus-based vector, they produced replication-defective virus at high titer but they did not release any replication-competent particles. Proviral DNA was shown to be correctly integrated as well as correctly expressed. Viral RNAs were shown to be correctly translated into gag-related polypeptides.

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Year:  1989        PMID： 2536089      PMCID： PMC247719     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  44 in total

1.  Generation of avian myeloblastosis virus structural proteins by proteolytic cleavage of a precursor polypeptide.

Authors:  V M Vogt; R Eisenman; H Diggelmann
Journal:  J Mol Biol       Date:  1975-08-15       Impact factor: 5.469

2.  Expression of endogenous ALV antigens and susceptibility to subgroup E ALV in three strains of chickens (endogenous avian C-type virus).

Authors:  H L Robinson; W F Lamoreux
Journal:  Virology       Date:  1976-01       Impact factor: 3.616

3.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

4.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

5.  RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.

Authors:  H Lehrach; D Diamond; J M Wozney; H Boedtker
Journal:  Biochemistry       Date:  1977-10-18       Impact factor: 3.162

6.  Identification of a large polypeptide precursor of avian oncornavirus proteins.

Authors:  V M Vogt; R Eisenman
Journal:  Proc Natl Acad Sci U S A       Date:  1973-06       Impact factor: 11.205

7.  Rapid transformation of cells by Rous sarcoma virus.

Authors:  H Hanafusa
Journal:  Proc Natl Acad Sci U S A       Date:  1969-06       Impact factor: 11.205

8.  Ribonucleic acid isolated by cesium chloride centrifugation.

Authors:  V Glisin; R Crkvenjakov; C Byus
Journal:  Biochemistry       Date:  1974-06-04       Impact factor: 3.162

9.  Detection of avian oncovirus group-specific antigens by the enzyme-linked immunosorbent assay.

Authors:  D P Clark; R M Dougherty
Journal:  J Gen Virol       Date:  1980-04       Impact factor: 3.891

10.  Continuous tissue culture cell lines derived from chemically induced tumors of Japanese quail.

Authors:  C Moscovici; M G Moscovici; H Jimenez; M M Lai; M J Hayman; P K Vogt
Journal:  Cell       Date:  1977-05       Impact factor: 41.582
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  13 in total

1.  Packaging cells for avian leukosis virus-based vectors with various host ranges.

Authors:  F L Cosset; C Ronfort; R M Molina; F Flamant; A Drynda; M Benchaibi; S Valsesia; V M Nigon; G Verdier
Journal:  J Virol       Date:  1992-09       Impact factor: 5.103

2.  Ectopic expression of the erythrocyte band 3 anion exchange protein, using a new avian retrovirus vector.

Authors:  S Fuerstenberg; H Beug; M Introna; K Khazaie; A Muñoz; S Ness; K Nordström; J Sap; I Stanley; M Zenke
Journal:  J Virol       Date:  1990-12       Impact factor: 5.103

3.  A new avian leukosis virus-based packaging cell line that uses two separate transcomplementing helper genomes.

Authors:  F L Cosset; C Legras; Y Chebloune; P Savatier; P Thoraval; J L Thomas; J Samarut; V M Nigon; G Verdier
Journal:  J Virol       Date:  1990-03       Impact factor: 5.103

4.  Homologous and nonhomologous retroviral recombinations are both involved in the transfer by infectious particles of defective avian leukosis virus-derived transcomplementing genomes.

Authors:  A Girod; A Drynda; F L Cosset; G Verdier; C Ronfort
Journal:  J Virol       Date:  1996-08       Impact factor: 5.103

Review 5.  A defective HIV-1 vector for gene transfer to human lymphocytes.

Authors:  C Parolin; J Sodroski
Journal:  J Mol Med (Berl)       Date:  1995-06       Impact factor: 4.599

Review 6.  Vectors derived from avian leukosis and sarcoma viruses.

Authors:  F Flamant; F L Cosset; J Samarut
Journal:  J Mol Med (Berl)       Date:  1995-04       Impact factor: 4.599

7.  The packaging phenotype of the SE21Q1b provirus is related to high proviral expression and not trans-acting factors.

Authors:  D J Anderson; J Stone; R Lum; M L Linial
Journal:  J Virol       Date:  1995-11       Impact factor: 5.103

8.  Immune response and resistance to Rous sarcoma virus challenge of chickens immunized with cell-associated glycoproteins provided with a recombinant avian leukosis virus.

Authors:  Y Chebloune; J Rulka; F L Cosset; S Valsesia; C Ronfort; C Legras; A Drynda; J Kuzmak; V M Nigon; G Verdier
Journal:  J Virol       Date:  1991-10       Impact factor: 5.103

9.  Efficiency and selectivity of RNA packaging by Rous sarcoma virus Gag deletion mutants.

Authors:  M Sakalian; J W Wills; V M Vogt
Journal:  J Virol       Date:  1994-09       Impact factor: 5.103

10.  Improvement of avian leukosis virus (ALV)-based retrovirus vectors by using different cis-acting sequences from ALVs.

Authors:  F L Cosset; C Legras; J L Thomas; R M Molina; Y Chebloune; C Faure; V M Nigon; G Verdier
Journal:  J Virol       Date:  1991-06       Impact factor: 5.103
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