Literature DB >> 25360182

Purification of Proteins From Cell-Culture Medium or Cell-Lysate by High-Speed Counter-Current Chromatography Using Cross-Axis Coil Planet Centrifuge.

Yoichi Shibusawa1, Yoichiro Ito2.   

Abstract

This review describes protein purifications from cell culture medium or cell-lysate by high speed counter-current chromatography using the cross-axis coil planet centrifuge. Purifications were performed using aqueous two phase systems composed of polyethylene glycols and dextrans.

Entities:  

Year:  2007        PMID： 25360182      PMCID： PMC4211269          DOI： 10.2174/1874065000701010028

Source DB:  PubMed          Journal:  Open Anal Chem J        ISSN： 1874-0650


  24 in total

1.  Counter-current chromatography of lipoproteins with a polymer phase system using the cross-axis synchronous coil planet centrifuge.

Authors:  Y Shibusawa; Y Ito; K Ikewaki; D J Rader; H B Brewer
Journal:  J Chromatogr       Date:  1992-04-03

Review 2.  Quantitative assays for characterization of the Sir2 family of NAD(+)-dependent deacetylases.

Authors:  Margie T Borra; John M Denu
Journal:  Methods Enzymol       Date:  2004       Impact factor: 1.600

3.  Improved high-speed counter-current chromatograph with three multilayer coils connected in series. IV. Evaluation of preparative capability with large multilayer coils.

Authors:  Y Ito; E Kitazume
Journal:  J Chromatogr       Date:  1991-01-18

4.  Protein separation with aqueous-aqueous polymer systems by two types of counter-current chromatographs.

Authors:  Y Shibusawa; Y Ito
Journal:  J Chromatogr       Date:  1991-07-26

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  Studies on a new cross-axis coil planet centrifuge for performing counter-current chromatography. I. Design of the apparatus, retention of the stationary phase, and efficiency in the separation of proteins with polymer phase systems.

Authors:  K Shinomiya; J M Menet; H M Fales; Y Ito
Journal:  J Chromatogr       Date:  1993-08-06

7.  Purification and properties of Streptococcus mutans extracellular glucosyltransferase.

Authors:  A Shimamura; H Tsumori; H Mukasa
Journal:  Biochim Biophys Acta       Date:  1982-03-18

8.  Purification of glucosyltransferase from cell-lysate of Streptococcus mutans by counter-current chromatography using aqueous polymer two-phase system.

Authors:  Akio Yanagida; Mitsuhiro Isozaki; Yoichi Shibusawa; Heisaburo Shindo; Yoichiro Ito
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2004-06-05       Impact factor: 3.205

9.  Purification of alcohol dehydrogenase from bovine liver crude extract by dye-ligand affinity counter-current chromatography.

Authors:  Yoichi Shibusawa; Takiko Fujiwara; Heisaburo Shindo; Yoichiro Ito
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2004-01-25       Impact factor: 3.205

10.  Purification of single-strand DNA binding protein from an Escherichia coli lysate using counter-current chromatography, partition and precipitation.

Authors:  Yoichi Shibusawa; Yohko Ino; Takashi Kinebuchi; Mitsuhiro Shimizu; Heisaburo Shindo; Yoichiro Ito
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2003-08-15       Impact factor: 3.205
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