Debjani Taraphdar1, Shyamalendu Chatterjee2. 1. ICMR Virus Unit, GB4, 1st floor, ID & BG Hospital, Dr. S.C. Banerjee Road, Beliaghata, Kolkata, India. 2. ICMR Virus Unit, GB4, 1st floor, ID & BG Hospital, Dr. S.C. Banerjee Road, Beliaghata, Kolkata, India shyamalendu.chatterjee1@gmail.com.
Abstract
BACKGROUND: In the state of West Bengal, chikungunya virus (CHIKV) has re-emerged in 2006 after its last occurrence in 1963-1965 in this state. The virus rapidly affected almost every district of this state, with high morbidity. Based on complete sequences of structural region of CHIKV genome, we determined the molecular characterization of the virus circulating in this state from 2006-2012. METHODS: CHIKV was isolated from 20 acute CHIKV RT-PCR positive serum samples in C6/36 mosquito cell line. These samples were collected from 20 patients with a clinical history of ≤2 days of fever and chikungunya-like illness. Those patients were residing in some outbreak areas in the state of West Bengal during 2006-2012. Isolation was confirmed through RT-PCR and sequencing. RESULTS AND CONCLUSIONS: Two sub-lineages of East-central-southern African (ECSA) genotype of CHIKV strains were circulating simultaneously in this state during the study period; one type was circulating in rural areas of the state from 2006 whereas another type was isolated from the metropolitan city of Kolkata in 2011 and 2012. The mutational pattern of those CHIKV strains suggests that the transmission of the viruses might be facilitated by different species of Aedes mosquitoes. Our results represent an important first step towards understanding the circulating strains of CHIKV in the state of West Bengal with the geographical variation.
BACKGROUND: In the state of West Bengal, chikungunya virus (CHIKV) has re-emerged in 2006 after its last occurrence in 1963-1965 in this state. The virus rapidly affected almost every district of this state, with high morbidity. Based on complete sequences of structural region of CHIKV genome, we determined the molecular characterization of the virus circulating in this state from 2006-2012. METHODS:CHIKV was isolated from 20 acute CHIKV RT-PCR positive serum samples in C6/36 mosquito cell line. These samples were collected from 20 patients with a clinical history of ≤2 days of fever and chikungunya-like illness. Those patients were residing in some outbreak areas in the state of West Bengal during 2006-2012. Isolation was confirmed through RT-PCR and sequencing. RESULTS AND CONCLUSIONS: Two sub-lineages of East-central-southern African (ECSA) genotype of CHIKV strains were circulating simultaneously in this state during the study period; one type was circulating in rural areas of the state from 2006 whereas another type was isolated from the metropolitan city of Kolkata in 2011 and 2012. The mutational pattern of those CHIKV strains suggests that the transmission of the viruses might be facilitated by different species of Aedes mosquitoes. Our results represent an important first step towards understanding the circulating strains of CHIKV in the state of West Bengal with the geographical variation.
Authors: Margarita V Rangel; Nicole McAllister; Kristen Dancel-Manning; Maria G Noval; Laurie A Silva; Kenneth A Stapleford Journal: J Virol Date: 2021-12-22 Impact factor: 6.549
Authors: Fredrick Eyase; Solomon Langat; Irina Maljkovic Berry; Francis Mulwa; Albert Nyunja; James Mutisya; Samuel Owaka; Samson Limbaso; Victor Ofula; Hellen Koka; Edith Koskei; Joel Lutomiah; Richard G Jarman; Rosemary Sang Journal: PLoS One Date: 2020-11-06 Impact factor: 3.240