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Literature DB >> 25357999

Development and characterization of three novel monoclonal antibodies against CA-125.

Tatiana Michurina¹, Maxim Kerzhner, Boris Klimovich.

Abstract

Cancer antigen 125 (CA-125) is the most widely used tumor marker for ovarian cancer. Thus, monoclonal antibodies (MAbs) against CA-125 are valuable reagents for the development of diagnostic tests and immunotherapy. We describe here the generation and characterization of three novel hybridoma cell lines producing MAbs against CA-125. CA-125 purified from culture supernatant of ovarian carcinoma cell line OVCAR-3 by affinity chromatography, was used for immunization of BALB/c mice. Three stable cell lines (3C8, 2B6, and 5A12) were selected for production of antibodies against CA-125 and were expanded in mass culture. All three antibodies were shown to recognize linear epitopes. Antibodies 2B6 and 5A12 were determined to recognize epitope cluster B (M 11-like); MAb 3C8 was classified as group A-epitope binders (OC 125-like). The antibodies produced may be used for the development and improvement of CA-125 immunoassays.

Entities: Disease Species

Mesh：

Substances：

Year: 2014 PMID： 25357999 PMCID： PMC4216484 DOI： 10.1089/mab.2014.0017

Source DB: PubMed Journal: Monoclon Antib Immunodiagn Immunother ISSN： 2167-9436

14 in total

1. The CA 125 gene: an extracellular superstructure dominated by repeat sequences.

Authors: T J O'Brien; J B Beard; L J Underwood; R A Dennis; A D Santin; L York
Journal: Tumour Biol Date: 2001 Nov-Dec

2. New monoclonal antibodies identify the glycoprotein carrying the CA 125 epitope.

Authors: T J O'Brien; L M Raymond; G A Bannon; D H Ford; H Hardardottir; F C Miller; J G Quirk
Journal: Am J Obstet Gynecol Date: 1991-12 Impact factor: 8.661

3. Specificity and affinity of 26 monoclonal antibodies against the CA 125 antigen: first report from the ISOBM TD-1 workshop. International Society for Oncodevelopmental Biology and Medicine.

Authors: K Nustad; R C Bast; T J Brien; O Nilsson; P Seguin; M R Suresh; T Saga; S Nozawa; O P Børmer; H W de Bruijn; M Nap; A Vitali; M Gadnell; J Clark; K Shigemasa; B Karlsson; F T Kreutz; D Jette; H Sakahara; K Endo; E Paus; D Warren; S Hammarström; P Kenemans; J Hilgers
Journal: Tumour Biol Date: 1996

4. Isolation and characterization of ovarian cancer antigen CA 125 using a new monoclonal antibody (VK-8): identification as a mucin-type molecule.

Authors: K O Lloyd; B W Yin; V Kudryashov
Journal: Int J Cancer Date: 1997-05-29 Impact factor: 7.396

5. Prognostic significance of CA 125 and TPS levels after 3 chemotherapy courses in ovarian cancer patients.

Authors: A van Dalen; J Favier; A Burges; U Hasholzner; H W de Bruijn; D Dobler-Girdziunaite; V H Dombi; D Fink; M Giai; P McGing; A Harlozinska; C Kainz; J Markowska; R Molina; C Sturgeon; A Bowman; R Einarsson
Journal: Gynecol Oncol Date: 2000-12 Impact factor: 5.482

Review 6. Kinetics of serum tumor marker concentrations and usefulness in clinical monitoring.

Authors: J M Bidart; F Thuillier; C Augereau; J Chalas; A Daver; N Jacob; F Labrousse; H Voitot
Journal: Clin Chem Date: 1999-10 Impact factor: 8.327

7. Production and characterization of monoclonal antibodies against the extracellular domain of CA 125.

Authors: Sorour Shojaeian; Abdolamir Allameh; Amir Hassan Zarnani; Mahmood Chamankhah; Roya Ghods; Ali Ahmad Bayat; Mahmood Jeddi-Tehrani
Journal: Immunol Invest Date: 2010-01 Impact factor: 3.657

8. Heterologous double-determinant immunoradiometric assay CA 125 II: reliable second-generation immunoassay for determining CA 125 in serum.

Authors: P Kenemans; G J van Kamp; P Oehr; R A Verstraeten
Journal: Clin Chem Date: 1993-12 Impact factor: 8.327