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Literature DB >> 2535525

A protein kinase from wheat germ that phosphorylates the largest subunit of RNA polymerase II.

Abstract

A protein kinase from wheat germ that phosphorylates the largest subunit of RNA polymerase IIA has been partially purified and characterized. The kinase has a native molecular weight of about 200 kilodaltons. This kinase utilizes Mg2+ and ATP and transfers about 20 phosphates to the heptapeptide repeats Pro-Thr-Ser-Pro-Ser-Tyr-Ser in the carboxyl-terminal domain of the 220-kilodalton subunit of soybean RNA polymerase II. This phosphorylation results in a mobility shift of the 220-kilodalton subunits of a variety of eukaryotic RNA polymerases to polypeptides ranging in size from greater than 220 kilodaltons to 240 kilodaltons on sodium dodecyl sulfate-polyacrylamide gels. The phosphorylation is highly specific to the heptapeptide repeats since a degraded subunit polypeptide of 180 kilodaltons that lacks the heptapeptide repeats is poorly phosphorylated. Synthetic heptapeptide repeat multimers inhibit the phosphorylation of the 220-kilodalton subunit.

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Year: 1989 PMID： 2535525 PMCID： PMC159820 DOI： 10.1105/tpc.1.8.827

Source DB: PubMed Journal: Plant Cell ISSN： 1040-4651 Impact factor: 11.277

31 in total

1. A new method for the large-scale purification of wheat germ DNA-dependent RNA polymerase II.

Authors: J J Jendrisak; R R Burgess
Journal: Biochemistry Date: 1975-10-21 Impact factor: 3.162

2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

3. In vitro proteolysis of a large subunit of Drosophila melanogaster RNA polymerase B.

Authors: A L Greenleaf; R Haars; E K Bautz
Journal: FEBS Lett Date: 1976-12-01 Impact factor: 4.124

4. The amounts, subunit structures, and template-engaged activities of RNA polymerases in germinating soybean axes.

Authors: T J Guilfoyle; S Malcolm
Journal: Dev Biol Date: 1980-07 Impact factor: 3.582

5. Phosphorylation of eukaryotic DNA-dependent RNA polymerase. Identification of calf thymus RNA polymerase subunits phosphorylated by two purified protein kinases, correlation with in vivo sites of phosphorylation in HeLa cell RNA polymerase II.

Authors: M E Dahmus
Journal: J Biol Chem Date: 1981-04-10 Impact factor: 5.157

6. Specific in vitro initiation of transcription on conalbumin and ovalbumin genes and comparison with adenovirus-2 early and late genes.

Authors: B Wasylyk; C Kédinger; J Corden; O Brison; P Chambon
Journal: Nature Date: 1980-06-05 Impact factor: 49.962

7. Selective and accurate initiation of transcription at the Ad2 major late promotor in a soluble system dependent on purified RNA polymerase II and DNA.

Authors: P A Weil; D S Luse; J Segall; R G Roeder
Journal: Cell Date: 1979-10 Impact factor: 41.582

8. Eukaryotic RNA polymerase: comparative subunit structures, immunological properties, and alpha-amanitin sensitivities of the class II enzymes from higher plants.

Authors: J Jendrisak; T J Guilfoyle
Journal: Biochemistry Date: 1978-04-04 Impact factor: 3.162

9. Auxin-induced deoxyribonucleic acid dependent ribonucleic acid polymerase activities in mature soybean hypocotyl.

Authors: T J Guilfoyle
Journal: Biochemistry Date: 1980-12-23 Impact factor: 3.162

10. Plant DNA-dependent RNA polymerases: subunit structures and enzymatic properties of the class II enzymes from quiescent and proliferating tissues.

Authors: T J Guilfoyle; J J Jendrisak
Journal: Biochemistry Date: 1978-05-16 Impact factor: 3.162

10 in total

1. Ku autoantigen is the regulatory component of a template-associated protein kinase that phosphorylates RNA polymerase II.

Authors: A Dvir; S R Peterson; M W Knuth; H Lu; W S Dynan
Journal: Proc Natl Acad Sci U S A Date: 1992-12-15 Impact factor: 11.205

10. Phosphorylation and dephosphorylation affect functional characteristics of chloroplast and etioplast transcription systems from mustard (Sinapis alba L.).

Authors: K Tiller; G Link
Journal: EMBO J Date: 1993-05 Impact factor: 11.598

10 in total

A protein kinase from wheat germ that phosphorylates the largest subunit of RNA polymerase II.

1. A new method for the large-scale purification of wheat germ DNA-dependent RNA polymerase II.

2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

3. In vitro proteolysis of a large subunit of Drosophila melanogaster RNA polymerase B.

4. The amounts, subunit structures, and template-engaged activities of RNA polymerases in germinating soybean axes.

5. Phosphorylation of eukaryotic DNA-dependent RNA polymerase. Identification of calf thymus RNA polymerase subunits phosphorylated by two purified protein kinases, correlation with in vivo sites of phosphorylation in HeLa cell RNA polymerase II.

6. Specific in vitro initiation of transcription on conalbumin and ovalbumin genes and comparison with adenovirus-2 early and late genes.

7. Selective and accurate initiation of transcription at the Ad2 major late promotor in a soluble system dependent on purified RNA polymerase II and DNA.

8. Eukaryotic RNA polymerase: comparative subunit structures, immunological properties, and alpha-amanitin sensitivities of the class II enzymes from higher plants.

9. Auxin-induced deoxyribonucleic acid dependent ribonucleic acid polymerase activities in mature soybean hypocotyl.

10. Plant DNA-dependent RNA polymerases: subunit structures and enzymatic properties of the class II enzymes from quiescent and proliferating tissues.

1. Ku autoantigen is the regulatory component of a template-associated protein kinase that phosphorylates RNA polymerase II.

2. Accurate in vitro transcription of plant promoters with nuclear extracts prepared from cultured plant cells.

3. Analysis of the genes encoding the largest subunit of RNA polymerase II in Arabidopsis and soybean.

4. Construction and analysis of yeast RNA polymerase II CTD deletion and substitution mutations.

5. Homologous domains of the largest subunit of eucaryotic RNA polymerase II are conserved in plants.

6. Purification and characterization of a casein kinase 2-type protein kinase from pea nuclei.

7. Multisite phosphorylation of 14-3-3 proteins by calcium-dependent protein kinases.

8. Isolation and partial characterization of a protein kinase NII from wheat germ chromatin.

9. CTD kinase large subunit is encoded by CTK1, a gene required for normal growth of Saccharomyces cerevisiae.

10. Phosphorylation and dephosphorylation affect functional characteristics of chloroplast and etioplast transcription systems from mustard (Sinapis alba L.).