Enhancement of post-anoxic denitrification for biological nutrient removal: effect of different carbon sources.

Previous research has demonstrated that post-anoxic denitrification and biological nutrient removal could be achieved in the oxic/anoxic/extended-idle wastewater treatment regime. This study further investigated the effect of different carbon sources on post-anoxic denitrification and biological nutrient removal. Acetate, propionate (volatile fatty acids (VFAs)), glucose (carbohydrate), methanol, and ethanol (alcohol) were used as the sole carbon source, respectively. The experimental results showed that VFA substrates led to an improvement in nitrogen and phosphorus removal. The total nitrogen and phosphorus removal efficiency values driven by acetate achieved 93 and 99%, respectively. In contrast, glucose present in mixed liquor deteriorated total nitrogen and phosphorus removal efficiency values to 72 and 54%. In the reactors cultured with methanol and ethanol, 66 and 63% of the total nitrogen were removed, and phosphorus removal efficiency values were 78 and 71%, respectively. The mechanism studies revealed that different carbon sources affected the transformations of intracellular polyhydroxyalkanoates (PHAs) and glycogen. PHAs are the dominant storages for microorganisms cultured with VFA substrates. Though glycogen is not the favorable energy and carbon source for polyphosphate-accumulating organisms, it can be consumed by microorganisms related to biological nitrogen removal and is able to serve as the electron donor for post-anoxic denitrification.