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Literature DB >> 25352174

Accurate microfluidic sorting of droplets at 30 kHz.

Abstract

Fluorescence-activated droplet sorting is an important tool for droplet microfluidic workflows, but published approaches are unable to surpass throughputs of a few kilohertz. We present a new geometry that replaces the hard divider separating the outlets with a gapped divider, allowing sorting over ten times faster.

Entities: Chemical Disease Species

Mesh：

Substances：
Fluorescent Dyes

Year: 2015 PMID： 25352174 PMCID： PMC4256106 DOI： 10.1039/c4lc01194e

Source DB: PubMed Journal: Lab Chip ISSN： 1473-0189 Impact factor: 6.799

19 in total

Review 1. Droplet microfluidics for high-throughput biological assays.

Authors: Mira T Guo; Assaf Rotem; John A Heyman; David A Weitz
Journal: Lab Chip Date: 2012-02-09 Impact factor: 6.799

2. A completely in vitro ultrahigh-throughput droplet-based microfluidic screening system for protein engineering and directed evolution.

Authors: Ali Fallah-Araghi; Jean-Christophe Baret; Michael Ryckelynck; Andrew D Griffiths
Journal: Lab Chip Date: 2012-01-26 Impact factor: 6.799

3. Experimental validation of plugging during drop formation in a T-junction.

Authors: Adam R Abate; Pascaline Mary; Volkert van Steijn; David A Weitz
Journal: Lab Chip Date: 2012-03-09 Impact factor: 6.799

4. Ultrahigh-throughput sorting of microfluidic drops with flow cytometry.

Authors: Shaun W Lim; Adam R Abate
Journal: Lab Chip Date: 2013-12-07 Impact factor: 6.799

5. Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity.

Authors: Jean-Christophe Baret; Oliver J Miller; Valerie Taly; Michaël Ryckelynck; Abdeslam El-Harrak; Lucas Frenz; Christian Rick; Michael L Samuels; J Brian Hutchison; Jeremy J Agresti; Darren R Link; David A Weitz; Andrew D Griffiths
Journal: Lab Chip Date: 2009-04-23 Impact factor: 6.799

6. Ultrahigh-throughput Mammalian single-cell reverse-transcriptase polymerase chain reaction in microfluidic drops.

Authors: Dennis J Eastburn; Adam Sciambi; Adam R Abate
Journal: Anal Chem Date: 2013-08-08 Impact factor: 6.986

7. Standing surface acoustic wave (SSAW) based multichannel cell sorting.

Authors: Xiaoyun Ding; Sz-Chin Steven Lin; Michael Ian Lapsley; Sixing Li; Xiang Guo; Chung Yu Chan; I-Kao Chiang; Lin Wang; J Philip McCoy; Tony Jun Huang
Journal: Lab Chip Date: 2012-11-07 Impact factor: 6.799

8. Single-cell analysis and sorting using droplet-based microfluidics.

Authors: Linas Mazutis; John Gilbert; W Lloyd Ung; David A Weitz; Andrew D Griffiths; John A Heyman
Journal: Nat Protoc Date: 2013-04-04 Impact factor: 13.491

9. Microfluidic high-throughput culturing of single cells for selection based on extracellular metabolite production or consumption.

Authors: Benjamin L Wang; Adel Ghaderi; Hang Zhou; Jeremy Agresti; David A Weitz; Gerald R Fink; Gregory Stephanopoulos
Journal: Nat Biotechnol Date: 2014-04-06 Impact factor: 54.908

10. One in a million: flow cytometric sorting of single cell-lysate assays in monodisperse picolitre double emulsion droplets for directed evolution.

Authors: Anastasia Zinchenko; Sean R A Devenish; Balint Kintses; Pierre-Yves Colin; Martin Fischlechner; Florian Hollfelder
Journal: Anal Chem Date: 2014-02-11 Impact factor: 6.986

54 in total

1. PCR-activated cell sorting as a general, cultivation-free method for high-throughput identification and enrichment of virus hosts.

Authors: Shaun W Lim; Shea T Lance; Kenneth M Stedman; Adam R Abate
Journal: J Virol Methods Date: 2016-12-29 Impact factor: 2.014

2. Dissecting enzyme function with microfluidic-based deep mutational scanning.

Authors: Philip A Romero; Tuan M Tran; Adam R Abate
Journal: Proc Natl Acad Sci U S A Date: 2015-05-26 Impact factor: 11.205

3. Ultrahigh-throughput-directed enzyme evolution by absorbance-activated droplet sorting (AADS).

Authors: Fabrice Gielen; Raphaelle Hours; Stephane Emond; Martin Fischlechner; Ursula Schell; Florian Hollfelder
Journal: Proc Natl Acad Sci U S A Date: 2016-11-07 Impact factor: 11.205

4. Activity-Based DNA-Encoded Library Screening.

Authors: Wesley G Cochrane; Marie L Malone; Vuong Q Dang; Valerie Cavett; Alexander L Satz; Brian M Paegel
Journal: ACS Comb Sci Date: 2019-03-29 Impact factor: 3.784

Review 5. Generation and manipulation of hydrogel microcapsules by droplet-based microfluidics for mammalian cell culture.

Authors: Haishui Huang; Yin Yu; Yong Hu; Xiaoming He; O Berk Usta; Martin L Yarmush
Journal: Lab Chip Date: 2017-05-31 Impact factor: 6.799

10. Label-Free On-Chip Selective Extraction of Cell-Aggregate-Laden Microcapsules from Oil into Aqueous Solution with Optical Sensor and Dielectrophoresis.

Authors: Mingrui Sun; Patrick Durkin; Jianrong Li; Thomas L Toth; Xiaoming He
Journal: ACS Sens Date: 2018-01-24 Impact factor: 7.711