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Literature DB >> 2535125

Synthetic model peptides phosphorylated in vitro by NII kinase and cation-mediated interactions with DNA.

G L Gianfranceschi¹, E Cardellini, G Piccinini, M Bramucci, A Miano, E Maccari, D Amici, E Durban.

Abstract

Minimum substrate requirements for nuclear NII kinase (casein II kinase) were analyzed with synthetic peptides modeled according to amino acid composition of phosphopeptides isolated from chromatin. Uncharged blocked peptide termini decreased the requirement for acidic clusters neighboring the phosphate acceptor amino acid (serine) such that only one group immediately N-terminal to serine was sufficient for kinase activity. Studies on peptide interaction with DNA showed that the model phosphopeptides bound to DNA only in the phosphorylated form suggesting involvement of phosphorylation in protein-DNA interactions yet to be identified.

Entities: Chemical

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Year: 1989 PMID： 2535125

Source DB: PubMed Journal: Physiol Chem Phys Med NMR ISSN： 0748-6642

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Cited

9 in total

1. Binding of small phosphorylated chromatin peptides to DNA.

Authors: E Cardellini; F Adami; G L Gianfranceschi
Journal: Mol Biol Rep Date: 1999-12 Impact factor: 2.316

2. Phosphorylation of the synthetic octapeptide pyroGlu-ASP-ASP-SER-ASP-GLU-GLU-ASN and binding to DNA in presence of divalent cations.

Authors: E Cardellini; D Ponti; G L Gianfranceschi
Journal: Mol Biol Rep Date: 1999-12 Impact factor: 2.316

9. Phosphorylation of synthetic acidic peptides by casein kinase II: evidence for competition with phosphorylation of proteins involved in transcription.

Authors: A Angiolillo; M Bramucci; V Marsili; F Panara; A Miano; D Amici; G L Gianfranceschi
Journal: Mol Cell Biochem Date: 1993-08-11 Impact factor: 3.396

9 in total

Synthetic model peptides phosphorylated in vitro by NII kinase and cation-mediated interactions with DNA.

1. Binding of small phosphorylated chromatin peptides to DNA.

2. Phosphorylation of the synthetic octapeptide pyroGlu-ASP-ASP-SER-ASP-GLU-GLU-ASN and binding to DNA in presence of divalent cations.

3. Small acidic peptides are bound to E. coli DNA.

4. Mass spectral and electrophoretic characterization of acidic peptides bound to chromatin of pea bud.

5. Competition between citrate and heptapeptide DDSDEEN binding to DNA in presence of divalent cations.

6. Synthetic octapeptide pyroGLU-ASP-ASP-SER-ASP-GLU-GLU-ASN controls DNA transcription in vitro by RNA polymerase II.

7. Phosphopeptides derived from in vitro phosphorylated E. coli RNA polymerase bind to DNA and affect DNA transcription.

8. Isolation and partial characterization of a protein kinase NII from wheat germ chromatin.

9. Phosphorylation of synthetic acidic peptides by casein kinase II: evidence for competition with phosphorylation of proteins involved in transcription.