Literature DB >> 25349920

Immunohistochemical and calcium imaging methods in wholemount rat retina.

Allison Sargoy1, Steven Barnes2, Nicholas C Brecha3, Luis Pérez De Sevilla Müller4.   

Abstract

In this paper we describe the tools, reagents, and the practical steps that are needed for: 1) successful preparation of wholemount retinas for immunohistochemistry and, 2) calcium imaging for the study of voltage gated calcium channel (VGCC) mediated calcium signaling in retinal ganglion cells. The calcium imaging method we describe circumvents issues concerning non-specific loading of displaced amacrine cells in the ganglion cell layer.

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Year:  2014        PMID： 25349920      PMCID： PMC4621789          DOI： 10.3791/51396

Source DB:  PubMed          Journal:  J Vis Exp        ISSN： 1940-087X            Impact factor:   1.355


  38 in total

Review 1.  Calcium signalling: dynamics, homeostasis and remodelling.

Authors:  Michael J Berridge; Martin D Bootman; H Llewelyn Roderick
Journal:  Nat Rev Mol Cell Biol       Date:  2003-07       Impact factor: 94.444

Review 2.  The ribonuclease protection assay: a powerful tool for the veterinary pathologist.

Authors:  J B Rottman
Journal:  Vet Pathol       Date:  2002-01       Impact factor: 2.221

3.  Calcium--a life and death signal.

Authors:  M J Berridge; M D Bootman; P Lipp
Journal:  Nature       Date:  1998-10-15       Impact factor: 49.962

4.  The major cell populations of the mouse retina.

Authors:  C J Jeon; E Strettoi; R H Masland
Journal:  J Neurosci       Date:  1998-11-01       Impact factor: 6.167

5.  Separation of calcium currents in retinal ganglion cells from postnatal rat.

Authors:  E Guenther; T Rothe; H Taschenberger; R Grantyn
Journal:  Brain Res       Date:  1994-01-07       Impact factor: 3.252

6.  Optical recordings of the effects of cholinergic ligands on neurons in the ganglion cell layer of mammalian retina.

Authors:  W H Baldridge
Journal:  J Neurosci       Date:  1996-08-15       Impact factor: 6.167

7.  Responses mediated by excitatory amino acid receptors in solitary retinal ganglion cells from rat.

Authors:  E Aizenman; M P Frosch; S A Lipton
Journal:  J Physiol       Date:  1988-02       Impact factor: 5.182

8.  Synaptic current kinetics in a solely AMPA-receptor-operated glutamatergic synapse formed by rat retinal ganglion neurons.

Authors:  H Taschenberger; F Engert; R Grantyn
Journal:  J Neurophysiol       Date:  1995-09       Impact factor: 2.714

9.  Permeation and block of N-methyl-D-aspartic acid receptor channels by divalent cations in mouse cultured central neurones.

Authors:  M L Mayer; G L Westbrook
Journal:  J Physiol       Date:  1987-12       Impact factor: 5.182

10.  The role of divalent cations in the N-methyl-D-aspartate responses of mouse central neurones in culture.

Authors:  P Ascher; L Nowak
Journal:  J Physiol       Date:  1988-05       Impact factor: 5.182
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  1 in total

1.  Prolonged Incubation of Acute Neuronal Tissue for Electrophysiology and Calcium-imaging.

Authors:  Morven A Cameron; Orsolya Kekesi; John W Morley; Alba Bellot-Saez; Sindy Kueh; Paul Breen; André van Schaik; Jonathan Tapson; Yossi Buskila
Journal:  J Vis Exp       Date:  2017-02-15       Impact factor: 1.355
  1 in total

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