| Literature DB >> 25348295 |
M Isabel Micalessi1, Gaëlle A Boulet, Johannes Bogers.
Abstract
A highly sensitive SPF10 real-time PCR was developed to achieve simultaneous amplification and detection of the human papillomavirus (HPV) target. That way, LiPA analysis of the HPV-negative samples can be avoided, reducing workload and cost. Here, we describe in detail a SYBR Green I-based real-time PCR assay based on SPF10 primers using the LightCycler(®) 480 system to generate and detect HPV amplicons, which are compatible with the LiPA assay.Entities:
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Year: 2015 PMID： 25348295 DOI： 10.1007/978-1-4939-2013-6_2
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745