The effect of storage media and duration of storage of extracted teeth on the shear bond strength of Scotchbond 2/Silux to dentin.

The objective was to determine the effect of storage media and the duration of storage of extracted teeth on the shear bond strength (SBS) of Scotchbond 2/Silux to dentin. Extracted human mandibular and maxillary first permanent molars were stored in buffered formalin (A), 1% chloramine (B), 70% ethanol (C), physiological saline (D), or 0.05% thymol (E). Fifteen test specimens were prepared on teeth stored in each medium two days after extraction (Procedures A1 to E1) or after six months storage (Procedures A2 to E2). The teeth were embedded in brass tooth cups and the occlusal surfaces ground wet on 180 and then 600 grit SiC immediately prior to the preparation of a test specimen. The Scotchprep Primer and the Scotchbond 2 Adhesive were applied to the dentin surfaces as directed by the manufacturer. Cylinders of Silux Composite were bonded to the treated dentin surfaces in a special device. The specimens were removed 15 minutes after cure and stored in distilled water at 37 degrees C for 24 hours. A shear load was applied to the bonded cylinders in an Instron machine at a crosshead speed of 0.02 inch.min-1. The SBS were calculated and expressed in MN.m-2. The data were analyzed by ANOVA and Duncan's multiple range test at the 5% level of significance.2+e mean SBS + SD