| Literature DB >> 25346525 |
Marcel Kwiatkowski1, Marcus Wurlitzer, Maryam Omidi, Ling Ren, Sebastian Kruber, Refat Nimer, Wesley D Robertson, Andrea Horst, R J Dwayne Miller, Hartmut Schlüter.
Abstract
A picosecond IR laser (PIRL) can be used to blast proteins out of tissues through desorption by impulsive excitation (DIVE) of intramolecular vibrational states of water molecules in the cell in less than a millisecond. With PIRL-DIVE proteins covering a range of a few kDa up to several MDa are extracted in high quantities compared to conventional approaches. The chemical composition of extracted proteins remains unaltered and even enzymatic activities are maintained.Entities:
Keywords: mass spectrometry; picosecond IR laser; protein extraction; proteomics; tissue samples
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Year: 2014 PMID： 25346525 DOI： 10.1002/anie.201407669
Source DB: PubMed Journal: Angew Chem Int Ed Engl ISSN： 1433-7851 Impact factor: 15.336