OBJECTIVES: Anti-cancer effects of melatonin (N-acetyl-5-methoxytryptamine, an indole-amine), have been widely reported, however, little has been known, regarding its mechanism(s) of action in lung cancer. Thus, we investigated its induction of apoptosis through biomolecular changes (lipid, protein and nucleic acid/DNA) in the SK-LU-1 human lung cancer cell line. MATERIALS AND METHODS: We used Fourier transform infrared (FTIR) microspectroscopy, and conventional methods, to confirm changes in lipid (annexin V/PI staining for membrane alteration), protein (caspase-3/7 protein activity) and DNA (DAPI staining for DNA fragmentation). RESULTS: We observed from FTIR data that melatonin increased lipid content and reduced intensity of nucleic acid/DNA, confirmed by annexin V/PI and DAPI respectively. Secondary protein structure at 1656 cm(-1) (α-helix) was reduced and peak position of β-sheet structure (1637 cm(-1) ) was shifted to lower frequency. Alteration in apoptotic proteins was demonstrated via caspase-3/7 activity induction. CONCLUSIONS: High melatonin concentration exerted anti-cancer effects by changing biomolecular structure of lipids, nucleic acids and proteins, supporting its enhancement of apoptotic induction.
OBJECTIVES: Anti-cancer effects of melatonin (N-acetyl-5-methoxytryptamine, an indole-amine), have been widely reported, however, little has been known, regarding its mechanism(s) of action in lung cancer. Thus, we investigated its induction of apoptosis through biomolecular changes (lipid, protein and nucleic acid/DNA) in the SK-LU-1 humanlung cancer cell line. MATERIALS AND METHODS: We used Fourier transform infrared (FTIR) microspectroscopy, and conventional methods, to confirm changes in lipid (annexin V/PI staining for membrane alteration), protein (caspase-3/7 protein activity) and DNA (DAPI staining for DNA fragmentation). RESULTS: We observed from FTIR data that melatonin increased lipid content and reduced intensity of nucleic acid/DNA, confirmed by annexin V/PI and DAPI respectively. Secondary protein structure at 1656 cm(-1) (α-helix) was reduced and peak position of β-sheet structure (1637 cm(-1) ) was shifted to lower frequency. Alteration in apoptotic proteins was demonstrated via caspase-3/7 activity induction. CONCLUSIONS: High melatonin concentration exerted anti-cancer effects by changing biomolecular structure of lipids, nucleic acids and proteins, supporting its enhancement of apoptotic induction.
Authors: R J Reiter; S Rosales-Corral; A Coto-Montes; J A Boga; D-X Tan; J M Davis; P C Konturek; S J Konturek; T Brzozowski Journal: J Physiol Pharmacol Date: 2011-06 Impact factor: 3.011
Authors: Alejandro Alvarez-Artime; Rafael Cernuda-Cernuda; Vanesa Cepas; Pedro Gonzalez-Menendez; Sheila Fernadez-Vega; Isabel Quiros-Gonzalez; Rosa M Sainz; Juan C Mayo Journal: Int J Mol Sci Date: 2020-01-15 Impact factor: 6.208