Literature DB >> 2534353

Convenient uses of polymerase chain reaction in analyzing recombinant cDNA clones.

B K Nishikawa1, D M Fowlkes, B K Kay.   

Abstract

We have used polymerase chain reaction to accelerate our analysis of recombinant lambda-cDNA clones. We have amplified the inserts of lambda gt10 or lambda gt11 recombinants starting with bacteriophage in cored plaques or isolated DNA. The amplifications made with simple or complex oligonucleotide primers have allowed convenient sizing, subcloning and translation of the phage inserts into protein.

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Year:  1989        PMID： 2534353

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  4 in total

1.  Gene activation is required for developmentally programmed cell death.

Authors:  L M Schwartz; L Kosz; B K Kay
Journal:  Proc Natl Acad Sci U S A       Date:  1990-09       Impact factor: 11.205

2.  Isolation of chalcone synthase and chalcone isomerase cDNAs from alfalfa (Medicago sativa L.): highest transcript levels occur in young roots and root tips.

Authors:  H I McKhann; A M Hirsch
Journal:  Plant Mol Biol       Date:  1994-03       Impact factor: 4.076

3.  Molecular cloning of the human kidney differentiation antigen gp160: human aminopeptidase A.

Authors:  D M Nanus; D Engelstein; G A Gastl; L Gluck; M J Vidal; M Morrison; C L Finstad; N H Bander; A P Albino
Journal:  Proc Natl Acad Sci U S A       Date:  1993-08-01       Impact factor: 11.205

4.  A tale of three kingdoms: members of the Phylum Nematoda independently acquired the detoxifying enzyme cyanase through horizontal gene transfer from plants and bacteria.

Authors:  D S Zarlenga; M Mitreva; P Thompson; R Tyagi; W Tuo; E P Hoberg
Journal:  Parasitology       Date:  2018-10-10       Impact factor: 3.234
  4 in total

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