Literature DB >> 2534351

Sequencing of cloned DNA using bacteriophage lambda gt11 templates.

D L Steffens1, R W Gross.   

Abstract

A procedure for isolating and directly sequencing recombinant bacteriophage lambda gt11 DNA templates is described. Approximately 250-300 bases of sequence can be obtained directly from the lambda gt11 template, eliminating the need for subcloning prior to dideoxynucleotide sequencing of clones.

Entities:  

Mesh:

Substances:

Year:  1989        PMID: 2534351

Source DB:  PubMed          Journal:  Biotechniques        ISSN: 0736-6205            Impact factor:   1.993


  5 in total

1.  Stimulation of polydnavirus replication by 20-hydroxyecdysone.

Authors:  B A Webb; M D Summers
Journal:  Experientia       Date:  1992-10-15

2.  Age-dependent induction of pyruvate, orthophosphate dikinase in Mesembryanthemum crystallinum L.

Authors:  B Fisslthaler; G Meyer; H J Bohnert; J M Schmitt
Journal:  Planta       Date:  1995       Impact factor: 4.116

3.  A truncated, secreted form of the epidermal growth factor receptor is encoded by an alternatively spliced transcript in normal rat tissue.

Authors:  L A Petch; J Harris; V W Raymond; A Blasband; D C Lee; H S Earp
Journal:  Mol Cell Biol       Date:  1990-06       Impact factor: 4.272

4.  Expression of a cDNA clone encoding the haem-binding domain of Chlorella nitrate reductase.

Authors:  A C Cannons; N Iida; L P Solomonson
Journal:  Biochem J       Date:  1991-08-15       Impact factor: 3.857

5.  Mouse UDP-GlcNAc: dolichyl-phosphate N-acetylglucosaminephosphotransferase. Molecular cloning of the cDNA, generation of anti-peptide antibodies and chromosomal localization.

Authors:  B Rajput; J Ma; N Muniappa; L Schantz; S L Naylor; P A Lalley; I K Vijay
Journal:  Biochem J       Date:  1992-08-01       Impact factor: 3.857

  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.