Jing Feng Yin1, Yu Lin Ding1, Ying Huang2, Xiao Yan Tao2, Hao Li2, Peng Cheng Yu2, Xin Xin Shen2, Wen Tao Jiao2, Guo Dong Liang3, Qing Tang2, Feng Long Wang1. 1. College of Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot 010018, Inner Mongolia, China. 2. Key Laboratory for Medical Virology, Ministry of Health, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (IVDC, China CDC), Beijing 102206, China. 3. State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (IVDC, China CDC), Beijing 102206, China.
Abstract
OBJECTIVE: To characterize two strains of street rabies virus (RABV) isolated from the brain tissue of cattle from Inner Mongolia. Differences in the histopathological and ultrastructural changes in the brain tissue of infected mice were determined to reveal variation in the pathogenesis of infection between street rabies virus strains. METHODS: Ten-day-old mice were intracranially inoculated with one of three virus strains and brain tissue harvested when the mice were moribund. Various histopathological and ultrastructural markers of disease were then compared between the groups. RESULTS: Infection with the street virus strain CNM1101C resulted in severe neuronal dendrites damage, but only mild cell apoptosis, T lymphocyte infiltration and microglial activation. Infection with the other street virus strain, CNM1103C, was characterized by cell apoptosis, T lymphocyte infiltration and microglial activation as well as dendrites damage. However, in comparison, infection with the attenuated virus strain CTN caused severe T lymphocyte infiltration, microglial activation and cell apoptosis, but left the neuronal dendrites intact. CONCLUSION: The two street rabies virus strains isolated from cattle from Inner Mongolia had different levels of virulence and caused distinct pathological changes in infected mice. Therefore, we concluded that different pathogenic mechanisms exist between different RABV strains.
OBJECTIVE: To characterize two strains of street rabies virus (RABV) isolated from the brain tissue of cattle from Inner Mongolia. Differences in the histopathological and ultrastructural changes in the brain tissue of infectedmice were determined to reveal variation in the pathogenesis of infection between street rabies virus strains. METHODS: Ten-day-old mice were intracranially inoculated with one of three virus strains and brain tissue harvested when the mice were moribund. Various histopathological and ultrastructural markers of disease were then compared between the groups. RESULTS:Infection with the street virus strain CNM1101C resulted in severe neuronal dendrites damage, but only mild cell apoptosis, T lymphocyte infiltration and microglial activation. Infection with the other street virus strain, CNM1103C, was characterized by cell apoptosis, T lymphocyte infiltration and microglial activation as well as dendrites damage. However, in comparison, infection with the attenuated virus strain CTN caused severe T lymphocyte infiltration, microglial activation and cell apoptosis, but left the neuronal dendrites intact. CONCLUSION: The two street rabies virus strains isolated from cattle from Inner Mongolia had different levels of virulence and caused distinct pathological changes in infectedmice. Therefore, we concluded that different pathogenic mechanisms exist between different RABV strains.
Authors: E Kip; F Nazé; V Suin; T Vanden Berghe; A Francart; S Lamoral; P Vandenabeele; R Beyaert; S Van Gucht; M Kalai Journal: Cell Death Discov Date: 2017-03-06