| Literature DB >> 25332432 |
Wolfram Lorenzen1, Kenan A J Bozhüyük1, Niña S Cortina2, Helge B Bode3.
Abstract
Analysis of whole cell lipid extracts of bacteria by means of ultra-performance (UP)LC-MS allows a comprehensive determination of the lipid molecular species present in the respective organism. The data allow conclusions on its metabolic potential as well as the creation of lipid profiles, which visualize the organism's response to changes in internal and external conditions. Herein, we describe: i) a fast reversed phase UPLC-ESI-MS method suitable for detection and determination of individual lipids from whole cell lipid extracts of all polarities ranging from monoacylglycerophosphoethanolamines to TGs; ii) the first overview of a wide range of lipid molecular species in vegetative Myxococcus xanthus DK1622 cells; iii) changes in their relative composition in selected mutants impaired in the biosynthesis of α-hydroxylated FAs, sphingolipids, and ether lipids; and iv) the first report of ceramide phosphoinositols in M. xanthus, a lipid species previously found only in eukaryotes.Entities:
Keywords: ceramide phosphoinositols; electrospray ionization-mass spectrometry; ether lipids; lipid profiles; lipidomics; myxobacteria; reversed phase; ultra-performance liquid chromatography
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Year: 2014 PMID： 25332432 PMCID： PMC4242454 DOI： 10.1194/jlr.M054593
Source DB: PubMed Journal: J Lipid Res ISSN： 0022-2275 Impact factor: 5.922